青蒿琥酯；_大鼠；_关节炎；_凋亡____

青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子Fas/FasL及Bcl2/Bax表达影响的实验观察

http://www.100md.com 《时珍国医国药》 2006年第10期

青蒿琥酯；,大鼠；,关节炎；,凋亡,,青蒿琥酯；,大鼠；,关节炎；,凋亡,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的观察青蒿琥酯对佐剂性关节炎滑膜组织中凋亡因子Fas/FasL及Bcl2/Bax表达的影响。方法雄性Wistar大鼠60只随机分为6组，空白组、模型组(对照组)、青蒿琥酯I组(高剂量组)、青蒿琥酯Ⅱ组(低剂量组)、青蒿琥酯加甲氨喋呤组及甲氨喋呤组。除空白组外，各实验组用完全弗氏佐剂制备成佐剂性关节炎模型。记录用药干预后各组关节肿胀指数，免疫组化法检测滑膜组织中凋亡相关因子Fas/FasL，Bcl2及Bax的表达情况。结果各实验组关节肿胀指数明显降低，与对照组比较差异有统计学意义(P<0.01)。滑膜组织中Fas/FasL及Bax被上调，Bcl2被下调，与对照组比较差异有统计学意义(P<0.05)。结论实验证实了青蒿琥酯有诱导佐剂性关节炎病情缓解的作用，上调滑膜组织中Fas/FasL及Bax，下调Bcl2的表达而诱导滑膜细胞凋亡可能是其机制之一。

    关键词：青蒿琥酯；大鼠；关节炎；凋亡

    The Experimental Research on the Expression of Fas/FasL and Bcl2/Bax Correlated with Apoptosis in Synoviocyte of Adjuvant Arthritis with Artesunate

    CHEN Xianguo， SONG Xingfu， CUI Xiangjun*， PAN Lei， HOU Xiaoqiang

    (The First College of Clinical Medical Science, China Three Gorges University, Yichang, Hubei 443003 China; Yichang Central People's Hospital, Yichang, Hubei 443000 China)

    Abstract：ObjectiveTo observe the expression of Fas/FasL and Bcl2/Bax correlated with apoptosis in synoviocyte of adjuvant arthritis (AA) with artesunate. MethodsSixty wistar rats were randomly divided into six groups:normal group, model control group, artesunate group I(high dose), artesunate group II(low dose), artesunate plus MTX group, MTX group. Each group was modeled to adjuvant arthritis with complete Freund's. After treated with artesunate or artesunate plus MTX, the arthrosis index(AI), the expression of Fas/FasL ......

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