氟伐他汀对大鼠放射性肺损伤防治作用的实验研究
氟伐他汀；放射性肺损伤；形态学；TGF-β1,,],氟伐他汀；放射性肺损伤；形态学；TGF-β1,1材料与方法,2结果,讨论,[参考文献]
[摘要] 目的 观察氟伐他汀对放射性肺损伤的防治作用，为放射性肺炎和肺间质纤维化的治疗探索一种新的方法。方法 雌性SD大鼠50只随机分为正常对照组(C组)，单纯照射组(R组)和氟伐他汀防治组(F组)。治疗组于照射前7天以氟伐他汀20 mg/(kg·d)灌胃，另两组灌服等量生理盐水直至实验结束。照射组和治疗组给予直线加速器照射，照射剂量单次20 Gy/min，于照射后5、15、30、60天取肺组织作HE，Masson，甲苯胺蓝染色及透射电镜，免疫组化法测定TGF-β1的表达，HE及免疫组化染色以计算机图像分析系统测定，数据用重复测量多因素方差分析和T检验。 结果 治疗组大鼠肺炎和肺水肿比照射组明显减轻。照射组大鼠肺组织胶原纤维量比治疗组明显增多。照射组15、30、60天肥大细胞数量逐渐增多(P<0.01)，而治疗组减少(P<0.01)，电镜显示Ⅱ型肺泡细胞和毛细血管内皮细胞30天时照射组较治疗组明显损伤，照射组30、60天肺间质增厚(P<0.01)，治疗组减轻，照射组TGF-β1的表达在30、60天较治疗组明显增高(P<0.01)。结论 氟伐他汀能抑制放射性炎症和组织损伤，可用于预防和治疗放射性肺损伤。[关键词] 氟伐他汀；放射性肺损伤；形态学；TGF-β1
Preventive effects of fluvastatin on radiationinduced lung injuries in rats
YE Jiangfeng,QI Haowen,MENG Yulin,et al.Department of Respiratory Diseases,PLA General Hospital,Lanzhou Military Command,Urumqi 830000,China
[Abstract] Objective To assess the efficacy of fluvastatin for prevention and treatment of radiationinduced lung injuries in rat.To evaluate the possibility of using fluvastatin as a new therapy for pneumonia and pulmonary fibrosis induced by radiation. Methods 50 SD female rats were randomly divided into control,radiation group and treatment groups .Fluvastatin was given to rats in treatment groups at a dose of 20 mg/(kg·day) by gastrogavaging from seven days before radiation until the end of the experiment.The rats in other groups were gastrogavaged with the same amount of saline. Rats in the radiation group and treatment group were exposed to thoracic radiation at a dose of 20 Gy by linear accelerator. Lung tissue from each rat was obtained,fixed and examined by HE staining,masson staining,toluidine blue staining and transmission electron microscopy. Expression of TGFβ1 were measured by immunohistochemistry staining. The field of observation of HE staining and immunohistochemistry statining were measured by a computerized image analysis system. The data were analyzed by ANOVA and students t test. Results Pneumonia and pulmonary edema in rats of treatment group were much less severe than in radiation group.The amount of collagen fibers in lung tissue from rats of radiation group increased significantly compared to treatment group.The number of mast cells was increased gradually in radiation group on days 15,30,60(P<0.01),but decreased in treatment group(P<0.01).Electron microscopy showed that type Ⅱ pneumonocytes and lung capillary endothelial cells were much more severely injured in radiation group on day 30 than in treatment group.The area of mesenchyma was increased in radiation group on day 30 and day 60(P<0.01),and decreased in the treatment group(P<0.01). The expression of TGFβ1 was high in radiation group on day 30 and day 60 (P<0.01) than in treatment group on days 15,30 and 60(P<0.01). Conclusion Fluvastatin might inhibit radiationinduced inflammation and tissue damage and could be used to prevent and treat radiationinduced lung injuries. ......
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