中药天年饮对衰老大鼠睾丸生精细胞增殖及相关因素的作用
天年饮；,衰老；,睾丸；,增殖细胞核抗原；,端粒酶,,天年饮；,衰老；,睾丸；,增殖细胞核抗原；,端粒酶,1材料与方法,2结果,3讨论,参考文献：
摘要：目的研究中药天年饮对衰老大鼠睾丸生精细胞增殖及相关因素的影响。方法40只 SD大鼠随机分为4组，正常组、衰老模型组、天年饮用药组及阴性对照组，每组均为10只。D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。采用ELISA方法检测各组大鼠血清睾酮含量、SP免疫组化法检测睾丸生精细胞 PCNA、端粒酶的表达情况。结果衰老大鼠血清睾酮含量、睾丸PCNA阳性细胞率及生精细胞端粒酶的表达明显低于正常大鼠(P<0.05，P<0.01)。天年饮可明显提高衰老大鼠血清睾酮含量(用药组与模型组比较 P<0.05)、提高睾丸 PCNA阳性细胞率(用药组与模型组比较 P<0.01)及提高衰老大鼠端粒酶的表达。结论天年饮可明显改善衰老大鼠的生精功能，具有一定延缓衰老的作用。关键词：天年饮； 衰老； 睾丸； 增殖细胞核抗原； 端粒酶
Effects of Traditional Chinese Medicine Tiannianyin on PCNA Expression and Related Factors of Spermatogenic Cells in Testis of Aging Rats
XUE Jingfeng, SONG Chengjun,LI Jian,CHEN Zhihong
(The Department of Human Anatomy, Chengde Medical College, Chengde, Hebei 067000, China；Chengde Central Hospital 067000, China)
Abstract:ObjectiveTo observe the effects of traditional Chinese medicine Tiannianyin on PCNA expression and related factors of spermatogenic cells in testis of aging rats.Methods40 SD rats were applied in this study and divided into 4 groups at random: normal group, aging model group, Tiannianyin group, negative control group, and each group had 10 rats. The subacute aging model rats were made by injecting Dgal into abdominal cavity continuousl. ELISA was used to detect the testosterone content in serum, SP immunohistochemistry to observe the PCNA and telomerase expression of spermatogenic cells in testis.ResultsThe testosterone content in serum, the PCNA positive cell rate and the telomerase expression of aging rats were obviously lower than normal rat (P<0.05, P<0.01). Tiannianyin could increase the testosterone content in serum (P<0.05), the PCNA positive cell rate (P<0.01) and the telomerase expression obviously. Conclusion:Traditional Chinese medicine Tiannianyin can improve the spermatogenic functions of aging rats obviously, and has fairly antiaging effects. ......
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