新疆枸杞子对大鼠肝微粒体脂质过氧化损伤体外模型的影响
新疆枸杞子；,微粒体；,丙二醛；,抗氧化,,新疆枸杞子；,微粒体；,丙二醛；,抗氧化,关键词：新疆枸杞子；微粒体；丙二醛；抗氧化,1材料与方法,2结果,3讨论,参考文献：
摘要： 目的：探讨新疆枸杞子的抗氧化作用。 方法：提取大鼠肝微粒体，制备Fe2+半胱氨酸诱发的肝微粒体脂质过氧化损伤的体外模型，检测新疆枸杞子的20个样品在不同终反应浓度(100 μg/ml、50 μg/ml、10 μg/ml、1 μg/ml)时对丙二醛(MDA)含量变化的影响，观察样品清除活性氧的抗氧化作用。每个浓度重复实验3次，最后得出样品在每个浓度的MDA抑制率的平均值。结果：12号样品即大孔树脂柱95%乙醇洗脱部分的MDA抑制率>50%，得出3组IC50值，分别为37.8%、23.1%、33.1%，平均为31.3%。结论：新疆枸杞子的大孔树脂柱95%乙醇洗脱部分具有抗氧化作用。关键词： 新疆枸杞子； 微粒体； 丙二醛； 抗氧化
Effect of Fructus Lycii in Xinjiang on the peroxidation damage model in microsome
GONG Can, Rena·Kasimu, Yibulayin·Simayi, et al
(Department of Phytochemistry and Pharmacognosy, College of Pharmacy，Xinjiang Medical University, Urumqi 830054， China)
Abstract： Objective: To investigate the antioxidation action of Fructus Lycii in Xinjiang. Methods: Sprague Dawley rats were killed and then the livers were removed to isolate the microsome which can generate the reactive oxygen species in the presence of Fe2+Cysteine.In these peroxidative damage models, at different final concentrations (100 μg/ml、50 μg/ml、10 μg/ml、1 μg/ml), the contents of malondialdehyde (MDA) were examined for analyzing the antioxidative action of Fructus Lycii in Xinjiang. At each concentration, we tested three times, at last, we got the average inhibition ratio of MDA. Result: The inhibition ratio of MDA of the number twelfth sample which was 95% alcohol elutropic fraction of macroporous resin of Fructus Lycii was more than 50%. We got IC50. They were 37.8%, 23.1% and 33.1% respectively. The average IC50 was 31.3%. Conclusion: The results have indicated that 95% alcohol elutropic fraction of macroporous resin of Fructus Lycii in Xinjiang shows antioxidation. ......
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