虎杖；_白藜芦醇；_大孔树脂；_硅胶柱层析____

虎杖中白藜芦醇的提取与分离工艺研究

http://www.100md.com 《时珍国医国药》 2006年第8期

虎杖；,白藜芦醇；,大孔树脂；,硅胶柱层析,,虎杖；,白藜芦醇；,大孔树脂；,硅胶柱层析,1材料与仪器,2方法与结果,3讨论,参考文献：

     摘要：目的优选虎杖的白藜芦醇最佳提取工艺。方法①采用正交实验法，以白藜芦醇提取量为指标，高效液相色谱法(HPLC)测含量，优选提取工艺条件。②以大孔树脂富集及色谱法分离、纯化白藜芦醇。结果乙醇提取最佳工艺为10倍量的90%乙醇回流提取2次，1 h/次。经AB8树脂吸附富集，硅胶柱色谱纯化，可获得高纯度的白藜芦醇产品。结论该工艺可用于指导生产。

    关键词：虎杖；白藜芦醇；大孔树脂；硅胶柱层析

    Study on Extraction Process of Resveratrol in Polygonum cuspidatum

    XU Hanlin, FU Yihe, GAO Peng, XU Chengjun

    (Hubei College of Traditional Chinese Medicine,Wuhan 430061;Yichang Institue for Drug Control,Yichang 443007,China)

    Abstract:ObjectiveTo study the optimum extraction process of resveratrol in Polygonum cuspidatum.Methods①The optimum extraction process was investigated by orthogonal design and the index of the method was the contents of resveratrol.②The technology of macroporous adsorptive resin and chromatography was used in the separation and purification of resveratrol by HPLC.ResultsThe optimum condition of ethanol extraction:10 times amount of 90% ethanol and extracted 2 times,1 hour one time.The purity of the product was gained by the technology of resin AB8 adsorption and silica column chromatography purification.ConclusionThe optimum extraction process can direct production. ......

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