环氧化酶-2选择性抑制剂抑制人食管癌细胞的生长及其诱导凋亡
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金春亭, 鲁 蓓, 李海军, 李玉珍, 武 欣, 范 婕, 张林西
食管癌;Eca-109细胞;TE-13细胞;凋亡;增殖;环氧化酶-2;四甲基唑蓝法;流式细胞术;放射免疫分析,金春亭,李海军,李玉珍,武欣,范婕,张林西,鲁蓓,金春亭,通讯作者:,Effectsofcyclooxygenase-
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金春亭, 李海军, 李玉珍, 武欣, 范婕, 张林西, 河北北方学院医学院病理学教研室 河北省张家口市 075029
鲁蓓, 河北北方学院附属第二医院普外科 河北省张家口市 075100
金春亭, 学士, 副教授, 主要从事消化道肿瘤的研究.河北省自然科学基金资助课题, No. C2005000664
通讯作者: 张林西, 075029, 河北省张家口市长青路14号, 河北北方学院医学院病理学教研室. linxizhang@sohu.com
电话: 0313-8045177 传真: 0313-8032544
收稿日期: 2006-11-18 接受日期: 2006-12-01
Effects of cyclooxygenase-2 selective inhibitor NS-398 on the proliferation and apoptosis of human esophageal carcinoma cell lines
Chun-Ting Jin, Bei Lu, Hai-Jun Li, Yu-Zhen Li, Xin Wu, Jie Fan, Lin-Xi Zhang
Chun-Ting Jin, Hai-Jun Li, Yu-Zhen Li, Xin Wu, Jie Fan, Lin-Xi Zhang,Department of Pathology, Hebei North University, Zhangjiakou 075029, Hebei Province, China
Bei Lu,Department of Surgery, the Second Affiliated Hospital of Hebei North University, Zhangjiakou 075100, Hebei Province, China
Supported bythe Natural Science Foundation of Hebei Province, No. C2005000664
Correspondence to:Lin-Xi Zhang, Department of Pathology, Hebei North University, 14 Changqing Road, Zhangjiakou 075029, Hebei Province, China. linxizhang@sohu.com
Received:2006-11-18 Accepted:2006-12-01
Abstract
AIM: To investigate the biological effects of NS-398, a cyclooxygenase-2 (COX-2) selective inhibitor, on esophageal carcinoma cell lines and the possible mechanism.
METHODS: Esophageal carcinoma cell lines Eca-109 and TE-13 were cultured by routine method, and then treated with different concentrations of NS-398 (5, 10, 20, 40, and 80 μmol/L) for 24, 48 and 72 hours. The growths of Eca-109 and TE-13 cells were measured by MTT assay. The expression of COX-2, Bcl-2 and Bax in the cells were detected by flow cytometry (FCM). TUNEL and FCM methods were also used to detect the NS-398-induced apoptosis of the cells. The content of prostaglandin E2 in the supernatants of cell culture was measured by radioimmunoassay (RIA) ......
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