丹芍化纤胶囊对肝纤维化大鼠PDGFR-β和p-ERK1/2的影响
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史保生, 侯瑞芳, 吴 君, 程明亮
肝纤维化;PDGF-b受体;磷酸化ERK1,2;丹芍化纤胶囊史保生,侯瑞芳,吴君,程明亮.丹芍化纤胶囊对肝纤维化大鼠PDGFR-b和p-ERK1,2的影响. 世界华人消化杂志2007;15(6)574-579,史保生,侯瑞
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史保生, 侯瑞芳, 鹤壁市传染病医院肝炎科 河南省鹤壁市 458000
吴君, 程明亮, 贵阳医学院第一附属医院感染科 贵州省贵阳市 550004
史保生, 医学硕士, 主治医师, 主要从事病毒性肝炎的临床研究.
通讯作者: 吴君, 550004, 贵州省贵阳市, 贵阳医学院第一附属医院感染科. wuwuj@21cn.com
电话: 0851-6813394
收稿日期: 2006-11-18 接受日期: 2006-12-27
Effect of Danshao Huaxian capsule on the expression of platelet-derived growth factor receptor β and phosphorylation events of the ERK1/2 in rats with experimental hepatic fibrosis
Bao-Sheng Shi, Rui-Fang Hou, Jun Wu, Ming-Liang Cheng
Bao-Sheng Shi, Rui-Fang Hou, Department of Hepatitis, Hebi Hospital of Infectious Diseases, Hebi 458000, He’nan Province, China
Jun Wu, Ming-Liang Cheng, Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Corresponding to: Jun Wu, Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China.
wuwuj@21cn.com
Received: 2006-11-18 Accepted: 2006-12-27
AbstractAIM:To investigate the effects of Danshao Huaxian capsule (DSHX) on the expression of platelet-derived growth factor receptor b and phosphorylated ERK1/2 (p-ERK1/2) in rats with experimental hepatic fibrosis, and to explore the anti-fibrosis mechanism of DSHX.
METHODS: A total of 55 male Sprague Dawley rats were randomly divided into group A, B, and C. The rats in group A served as normal controls, and those in group B and C were treated with carbon tetrachloride, ethanol and high-fat diet to establish the model of liver fibrosis. Meanwhile, the rats in group C were intragastrically administered with 0.8 g/kg DSHX once per day for 8 weeks. HE staining was used to observe the pathological changes of liver tissues. The expression of PDGFR-band p-ERK1/2 in fibrosis tissues were detected by immunohistochemistry and Western blot, respectively. Biochemical examination were performed to determine the serum levels of hyaluronic acid (HA), laminin (LN), typeⅢ procollagen peptide (PⅢP), albumin (ALB), total protein (TP), and the ratio of albumin to globulin (A/G) was also calculated.
RESULTS:Pathological examination confirmed that the model of rat hepatic fibrosis was induced successfully at the end of the 8th week. As compared with those in group A, the expression of PDGFR-b,p-ERK1/2 in liver tissues, the serum levels of HA, LN, and PⅢP were significantly increased (PDGFR-b:184.6 ± 8.5 vs 89.6 ± 5.8, P < 0.05; p-ERK1/2: 360.0 ± 14.5 vs 15.4 ± 2.1, P < 0.05; HA: 517.5 ± 91.5mg/Lvs 254.4 ± 33.1mg/L,P < 0.05; LN: 58.4 ± 11.3mg/Lvs 37.3 ± 9.8mg/L,P < 0.05; PⅢP: 36.9 ± 5.6 mg/Lvs 4.7 ± 1.5 mg/L,P < 0.05) in group B, while the serum level of ALB and the value of A/G were markedly decreased (ALB: 27.4 ± 4.9 g/L vs 42.1 ± 1.6 g/L, P < 0.05; A/G: 0.89 ± 0.08 vs 1.38 ± 0.09, P < 0.05). However, in group C, the expression of PDGFR-b, p-ERK1/2 in liver tissues, the serum levels of HA, LN, and PⅢP were markedly decreased (PDGFR-b: 91 ......
史保生, 侯瑞芳, 鹤壁市传染病医院肝炎科 河南省鹤壁市 458000
吴君, 程明亮, 贵阳医学院第一附属医院感染科 贵州省贵阳市 550004
史保生, 医学硕士, 主治医师, 主要从事病毒性肝炎的临床研究.
通讯作者: 吴君, 550004, 贵州省贵阳市, 贵阳医学院第一附属医院感染科. wuwuj@21cn.com
电话: 0851-6813394
收稿日期: 2006-11-18 接受日期: 2006-12-27
Effect of Danshao Huaxian capsule on the expression of platelet-derived growth factor receptor β and phosphorylation events of the ERK1/2 in rats with experimental hepatic fibrosis
Bao-Sheng Shi, Rui-Fang Hou, Jun Wu, Ming-Liang Cheng
Bao-Sheng Shi, Rui-Fang Hou, Department of Hepatitis, Hebi Hospital of Infectious Diseases, Hebi 458000, He’nan Province, China
Jun Wu, Ming-Liang Cheng, Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
Corresponding to: Jun Wu, Department of Infectious Diseases, the Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China.
wuwuj@21cn.com
Received: 2006-11-18 Accepted: 2006-12-27
AbstractAIM:To investigate the effects of Danshao Huaxian capsule (DSHX) on the expression of platelet-derived growth factor receptor b and phosphorylated ERK1/2 (p-ERK1/2) in rats with experimental hepatic fibrosis, and to explore the anti-fibrosis mechanism of DSHX.
METHODS: A total of 55 male Sprague Dawley rats were randomly divided into group A, B, and C. The rats in group A served as normal controls, and those in group B and C were treated with carbon tetrachloride, ethanol and high-fat diet to establish the model of liver fibrosis. Meanwhile, the rats in group C were intragastrically administered with 0.8 g/kg DSHX once per day for 8 weeks. HE staining was used to observe the pathological changes of liver tissues. The expression of PDGFR-band p-ERK1/2 in fibrosis tissues were detected by immunohistochemistry and Western blot, respectively. Biochemical examination were performed to determine the serum levels of hyaluronic acid (HA), laminin (LN), typeⅢ procollagen peptide (PⅢP), albumin (ALB), total protein (TP), and the ratio of albumin to globulin (A/G) was also calculated.
RESULTS:Pathological examination confirmed that the model of rat hepatic fibrosis was induced successfully at the end of the 8th week. As compared with those in group A, the expression of PDGFR-b,p-ERK1/2 in liver tissues, the serum levels of HA, LN, and PⅢP were significantly increased (PDGFR-b:184.6 ± 8.5 vs 89.6 ± 5.8, P < 0.05; p-ERK1/2: 360.0 ± 14.5 vs 15.4 ± 2.1, P < 0.05; HA: 517.5 ± 91.5mg/Lvs 254.4 ± 33.1mg/L,P < 0.05; LN: 58.4 ± 11.3mg/Lvs 37.3 ± 9.8mg/L,P < 0.05; PⅢP: 36.9 ± 5.6 mg/Lvs 4.7 ± 1.5 mg/L,P < 0.05) in group B, while the serum level of ALB and the value of A/G were markedly decreased (ALB: 27.4 ± 4.9 g/L vs 42.1 ± 1.6 g/L, P < 0.05; A/G: 0.89 ± 0.08 vs 1.38 ± 0.09, P < 0.05). However, in group C, the expression of PDGFR-b, p-ERK1/2 in liver tissues, the serum levels of HA, LN, and PⅢP were markedly decreased (PDGFR-b: 91 ......
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