碱性成纤维细胞生长因子促进兔缺血后肢 血管新生的实验研究
缺血·成纤维细胞生长因子·新生血管化,生理性·兔,,缺血·成纤维细胞生长因子·新生血管化，生理性·兔,碱性成纤维细胞生长因子促进兔缺血后肢血管新生的实验研究,1材料和方法,2结果,3讨论,参考文献
【摘要】目的：了解局部应用碱性成纤维细胞生长因子(bFGF)对促进兔缺血后肢血管新生的作用。方法：25只日本大耳白兔随机分2组，外科结扎切断各兔股动脉及其分支，制作兔后肢缺血模型。试验组各兔在缺血后肢肌肉内多次注射重组人bFGF蛋白(n=15)；对照组给予等剂量生理盐水(n=10)。术后4周，各兔行腹主动脉造影观察侧支血管形成情况，取内收肌和腓肠肌肌肉行病理切片HE染色应用图像分析系统统计血管密度，并用免疫组织化学方法检测内收肌和腓肠肌中VEGF阳性表达的血管数。结果：试验组兔侧支循环血管条数、血管密度及VEGF阳性表达的血管数均大于对照组(P<0.01)，缺血状态得到改善。结论：在兔缺血后肢肌肉中注射bFGF蛋白可促进血管新生，增加兔缺血后肢血液灌注，改善肢体缺血状态。【关键词】缺血·成纤维细胞生长因子·新生血管化，生理性·兔
Basic fibroblast growth factor(bFGF) improves the angiogenesis of the ischemic limbs in rabbits
MA Xiuxian1,WANG Zhiwei2,LI Feng2
1Depatment of General Surgery,2Department of Gynecology and Obstertrics,the First
Affiliated Hospital ,Zhengzhou University(Henan 450052,China)
【ABSTRACT】Objective:To evaluate the effect of local application bFGF on angiogenesis in ischemic limbs of rabbits.Methods:After ischemia models were induced by surgical ligation and cutting off left femoral artery in each rabbit,rabbits were randomized to received intramuscular injection of recombinant human bFGF(n=15)or normal saline(n=10)in ischemic zone.Four weeks later, angiogenesis of ischemia models were observed and evaluated by abdominal aortography,histopathologic analysis and immunohistochemical studies with the antibodies of VEGF.Results:The number of capillaries,the compensatory circulation blood vessel and the vascular endothelial cells that expressed VEGF protein in bFGF group is more than those of saline group(P<0.01).Conclusion:Intramuscular injection of recombinant human bFGF can improve the angiogenesis of the ischemic limbs is rabbits. ......
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