肠化胃黏膜病变组织HSP60的表达与幽门螺杆菌感染的相关性
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刘东屏, 吕晓辉, 张艳梅, 王炳元
热休克蛋白60;肠化胃黏膜;胃癌;幽门螺杆菌,刘东屏,吕晓辉,张艳梅,王炳元,通讯作者:,Expressionofheatshockprotein60ingastricmucosawithintestinalmetaplasiaand
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刘东屏, 吕晓辉, 张艳梅, 王炳元, 中国医科大学附属第一医院消化内科 辽宁省沈阳市 110001
通讯作者: 刘东屏, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院消化内科. dpl19632003@yahoo.com.cn
电话: 024-83282199
收稿日期: 2006-11-14 接受日期: 2006-12-18
Expression of heat shock protein 60 in gastric mucosa with intestinal metaplasia and its correlation with Helicobacter pylori infection
Dong-Ping Liu, Xiao-Hui Lv, Yan-Mei Zhang, Bing-Yuan Wang
Dong-Ping Liu, Xiao-Hui Lv, Yan-Mei Zhang, Bing-Yuan Wang,Department of Gastroenterology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to:Dong-Ping Liu, Department of Gastroenterology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. dpl19632003@yahoo.com.cn
Received:2006-11-14 Accepted:2006-12-18
Abstract
AIM: To investigate the expression of heat shock protein 60 (HSP60) and H pylori infection in gastric mucosa with intestinal metaplasia (IM).
METHODS: A total of 171 IM mucosa (54, 76, and 41 cases for type Ⅰ, Ⅱ, and Ⅲ, respectively) and 89 controls (40 and 49 cases for superficial gastritis and gastric cancer, respectively) were included in this study. SP immunohistochemisty was used to detect the expression of HSP60 and H pylori infection was judged by enzyme-linked immunosorbnent assay (ELISA), anti-H pylori IgG detection and H pylori -DNA polymerase chain reaction (PCR), respectively.
RESULTS: HSP60 expression was moderately positive in gastric mucosa with typeⅠ and Ⅱ and Ⅲ IM; the positive rate was 35.19% and 51.32% and 75.61%, and the over-expression rate was 1.9%, 3.9% and 19.5%, respectively. HSP60 expression was weak or moderately positive in superficial gastritis, and the positive rate was 30.00%. The positive rate of HSP60 expression in IM mucosa was increased statistically as compared with that in superficial gastritis (P < 0.01). Moderately or strongly positive expression of HSP60 was predominant (73.47%) in gastric cancer, and the over-expression rate was 34.69%, which was markedly higher than that in type Ⅰand type Ⅱ IM. There was a negative relationship between HSP60 expression and H pylori infection of IM mucosa (r = -0.191, P = 0.012).
CONCLUSION: HSP60 over-expression occurs in the progression of gastric mucosal lesions, and H pylori infection of IM mucosa is associated with HSP60 expression.
Key Words: Heat shock protein 60; Intestinal metaplasia; Gastric cancer; Helicobacter pylori
Liu DP, Lv XH, Zhang YM, Wang BY. Expression of heat shock protein 60 in gastric mucosa with intestinal metaplasia and its correlation with Helicobacter pylori infection. Shijie Huaren Xiaohua Zazhi 2007;15(7):758-761
摘要
目的: 探讨HSP60在肠化胃黏膜组织中的表达及与H pylori感染之间的关系.
方法: 对171例肠化胃黏膜组织其中Ⅰ型肠化54例、Ⅱ型肠化76例、Ⅲ型肠化41例, 对照组浅表性胃炎40例, 胃癌49例, 采用SP免疫组织化学技术进行胃黏膜HSP60的检测, 用ELISA, H pylori IgG抗体检测及H pylori-DNA PCR方法进行H pylori感染情况的判定 ......
刘东屏, 吕晓辉, 张艳梅, 王炳元, 中国医科大学附属第一医院消化内科 辽宁省沈阳市 110001
通讯作者: 刘东屏, 110001, 辽宁省沈阳市, 中国医科大学附属第一医院消化内科. dpl19632003@yahoo.com.cn
电话: 024-83282199
收稿日期: 2006-11-14 接受日期: 2006-12-18
Expression of heat shock protein 60 in gastric mucosa with intestinal metaplasia and its correlation with Helicobacter pylori infection
Dong-Ping Liu, Xiao-Hui Lv, Yan-Mei Zhang, Bing-Yuan Wang
Dong-Ping Liu, Xiao-Hui Lv, Yan-Mei Zhang, Bing-Yuan Wang,Department of Gastroenterology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China
Correspondence to:Dong-Ping Liu, Department of Gastroenterology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China. dpl19632003@yahoo.com.cn
Received:2006-11-14 Accepted:2006-12-18
Abstract
AIM: To investigate the expression of heat shock protein 60 (HSP60) and H pylori infection in gastric mucosa with intestinal metaplasia (IM).
METHODS: A total of 171 IM mucosa (54, 76, and 41 cases for type Ⅰ, Ⅱ, and Ⅲ, respectively) and 89 controls (40 and 49 cases for superficial gastritis and gastric cancer, respectively) were included in this study. SP immunohistochemisty was used to detect the expression of HSP60 and H pylori infection was judged by enzyme-linked immunosorbnent assay (ELISA), anti-H pylori IgG detection and H pylori -DNA polymerase chain reaction (PCR), respectively.
RESULTS: HSP60 expression was moderately positive in gastric mucosa with typeⅠ and Ⅱ and Ⅲ IM; the positive rate was 35.19% and 51.32% and 75.61%, and the over-expression rate was 1.9%, 3.9% and 19.5%, respectively. HSP60 expression was weak or moderately positive in superficial gastritis, and the positive rate was 30.00%. The positive rate of HSP60 expression in IM mucosa was increased statistically as compared with that in superficial gastritis (P < 0.01). Moderately or strongly positive expression of HSP60 was predominant (73.47%) in gastric cancer, and the over-expression rate was 34.69%, which was markedly higher than that in type Ⅰand type Ⅱ IM. There was a negative relationship between HSP60 expression and H pylori infection of IM mucosa (r = -0.191, P = 0.012).
CONCLUSION: HSP60 over-expression occurs in the progression of gastric mucosal lesions, and H pylori infection of IM mucosa is associated with HSP60 expression.
Key Words: Heat shock protein 60; Intestinal metaplasia; Gastric cancer; Helicobacter pylori
Liu DP, Lv XH, Zhang YM, Wang BY. Expression of heat shock protein 60 in gastric mucosa with intestinal metaplasia and its correlation with Helicobacter pylori infection. Shijie Huaren Xiaohua Zazhi 2007;15(7):758-761
摘要
目的: 探讨HSP60在肠化胃黏膜组织中的表达及与H pylori感染之间的关系.
方法: 对171例肠化胃黏膜组织其中Ⅰ型肠化54例、Ⅱ型肠化76例、Ⅲ型肠化41例, 对照组浅表性胃炎40例, 胃癌49例, 采用SP免疫组织化学技术进行胃黏膜HSP60的检测, 用ELISA, H pylori IgG抗体检测及H pylori-DNA PCR方法进行H pylori感染情况的判定 ......
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