不同地区幽门螺杆菌cagA基因羧基端可变区及其蛋白序列差异分析
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吴 莺, 张尤历, 王文兵, 陈劲频, 沈 琰
幽门螺杆菌;细胞毒性相关基因A;EPIYA基序;差异,吴莺,张尤历,陈劲频,沈琰,王文兵,通讯作者:,DiversityofcagAgene3’terminalvariableregionanditscorrespondingp
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吴莺, 张尤历, 陈劲频, 沈琰, 江苏大学附属医院消化科 江苏省镇江市 212001
王文兵, 江苏大学生命科学院 江苏省镇江市 212001
江苏省科学技术厅社会发展项目, No. BS2002023
通讯作者: 张尤历, 212001, 江苏省镇江市解放路438号, 江苏大学附属医院消化科. zjyouli@yahoo.com.cn
电话: 0511-5011787
收稿日期: 2006-09-28 接受日期: 2007-01-04
Diversity of cagA gene 3’ terminal variable region and its corresponding protein in Helicobacter pylori
Ying Wu, You-Li Zhang, Wen-Bing Wang, Jin-Pin Chen, Yan Shen
Ying Wu, You-Li Zhang, Jin-Pin Chen, Yan Shen,Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Wen-Bing Wang,Institute of Life Science, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Supported bythe Social Development Program of Jiangsu Science and Technology Department, No.BS2002023
Correspondence to:You-Li Zhang, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, Jiangsu Province, China. zjyouli@yahoo.com.cn
Received:2006-09-28 Accepted:2007-01-04
Abstract
AIM: To study the diversity and structural characteristics of cagA (cytotoxin-associated gene A) gene 3’ terminal variable region inHelicobacter pylori as well as their correlations with the outcome of H pylori-related diseases.
METHODS: PCR products with positive H pylori cagA were obtained from 20 cases and then sequenced by ExPASy-Translation software. Meanwhile, 3 to 6 cases of H pylori 3’ terminal variable region and amino acid sequence, collected from NCBI database in different countries, were comparatively analyzed.
RESULTS: The amino acid sequences were found with obvious regional characteristics, and could be classified into eastern-asian type and western type. Western-type aberrations occurred in some eastern-asian strains. Totally, 87.4% H pylori stains contained 3 EPIYA motifs in series, and 8.2% strains had 4 or more EPIYA motifs. A new sequence containing 3 amino acids was found. There was no correlation between the numbers of EPIYA motifs in the amino acid sequences of cagA protein and the clinical outcomes.
CONCLUSION: There exists marked diversity in cagA gene and protein of H pylori, which can be distinguished by the main sequences of H pylori cagA 3’ terminal variable region. The number of EPIYA motifs in cagA 3’ terminal variable region is not correlated with the clinical outcomes of diseases.
Key Words: Helicobacter pylori; Cytotoxin-associated gene A; EPIYA motif; Diversity
Wu Y, Zhang YL, Wang WB, Chen JP, Shen Y. Diversity of cagA gene 3’ terminal variable region and its corresponding protein in Helicobacter pylori. Shijie Huaren Xiaohua Zazhi 2007;15(7):746-749
摘要
目的: 比较不同地区H pylori cagA 3'端可变区序列差异及序列的地域特征, 分析差异与疾病的相关性.
方法: 选择本实验室所收集的20例cagA+的PCR产物进行测序, 并通过ExPASy-Translation软件推测其氨基酸序列, 搜索并收集GenBank中已公布的不同地区3-6个H pylori cagA 3'端可变区序列及其氨基酸序列进行比较分析 ......
吴莺, 张尤历, 陈劲频, 沈琰, 江苏大学附属医院消化科 江苏省镇江市 212001
王文兵, 江苏大学生命科学院 江苏省镇江市 212001
江苏省科学技术厅社会发展项目, No. BS2002023
通讯作者: 张尤历, 212001, 江苏省镇江市解放路438号, 江苏大学附属医院消化科. zjyouli@yahoo.com.cn
电话: 0511-5011787
收稿日期: 2006-09-28 接受日期: 2007-01-04
Diversity of cagA gene 3’ terminal variable region and its corresponding protein in Helicobacter pylori
Ying Wu, You-Li Zhang, Wen-Bing Wang, Jin-Pin Chen, Yan Shen
Ying Wu, You-Li Zhang, Jin-Pin Chen, Yan Shen,Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Wen-Bing Wang,Institute of Life Science, Jiangsu University, Zhenjiang 212001, Jiangsu Province, China
Supported bythe Social Development Program of Jiangsu Science and Technology Department, No.BS2002023
Correspondence to:You-Li Zhang, Department of Gastroenterology, the Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang 212001, Jiangsu Province, China. zjyouli@yahoo.com.cn
Received:2006-09-28 Accepted:2007-01-04
Abstract
AIM: To study the diversity and structural characteristics of cagA (cytotoxin-associated gene A) gene 3’ terminal variable region inHelicobacter pylori as well as their correlations with the outcome of H pylori-related diseases.
METHODS: PCR products with positive H pylori cagA were obtained from 20 cases and then sequenced by ExPASy-Translation software. Meanwhile, 3 to 6 cases of H pylori 3’ terminal variable region and amino acid sequence, collected from NCBI database in different countries, were comparatively analyzed.
RESULTS: The amino acid sequences were found with obvious regional characteristics, and could be classified into eastern-asian type and western type. Western-type aberrations occurred in some eastern-asian strains. Totally, 87.4% H pylori stains contained 3 EPIYA motifs in series, and 8.2% strains had 4 or more EPIYA motifs. A new sequence containing 3 amino acids was found. There was no correlation between the numbers of EPIYA motifs in the amino acid sequences of cagA protein and the clinical outcomes.
CONCLUSION: There exists marked diversity in cagA gene and protein of H pylori, which can be distinguished by the main sequences of H pylori cagA 3’ terminal variable region. The number of EPIYA motifs in cagA 3’ terminal variable region is not correlated with the clinical outcomes of diseases.
Key Words: Helicobacter pylori; Cytotoxin-associated gene A; EPIYA motif; Diversity
Wu Y, Zhang YL, Wang WB, Chen JP, Shen Y. Diversity of cagA gene 3’ terminal variable region and its corresponding protein in Helicobacter pylori. Shijie Huaren Xiaohua Zazhi 2007;15(7):746-749
摘要
目的: 比较不同地区H pylori cagA 3'端可变区序列差异及序列的地域特征, 分析差异与疾病的相关性.
方法: 选择本实验室所收集的20例cagA+的PCR产物进行测序, 并通过ExPASy-Translation软件推测其氨基酸序列, 搜索并收集GenBank中已公布的不同地区3-6个H pylori cagA 3'端可变区序列及其氨基酸序列进行比较分析 ......
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