罗格列酮对日本血吸虫病肝纤维化小鼠肝组织核因子-κB和过氧化物酶体增殖物激活受体γ表达的影响
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谌 辉, 张景辉, 刘文琪, 贺永文
罗格列酮;日本血吸虫病;核因子-κB;过氧化物酶体增殖物激活受体γ,谌辉,贺永文,张景辉,刘文琪,通讯作者:,Effectofrosiglitazoneontheactivityofhepati
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谌辉, 贺永文, 华中科技大学同济医学院附属协和医院传染科 湖北省武汉市 430030
张景辉, 华中科技大学同济医学院附属协和医院外科实验室 湖北省武汉市 430030
刘文琪, 华中科技大学同济医学院寄生虫学教研室 湖北省武汉市 430022
湖北省自然科学基金资助项目, No. 2005ABA170
通讯作者: 谌辉, 430030, 湖北省武汉市, 华中科技大学同济医学院附属协和医院传染科. chenhui0515@yahoo.com.cn
电话: 027-85726419
收稿日期: 2006-11-20 接受日期: 2006-12-27
Effect of rosiglitazone on the activity of hepatic nuclear factor-kappa B and expression of peroxisome proliferator-activated receptor-γ in mice with liver fibrosis due to schistosoma japonicum infection
Hui Chen, Jing-Hui Zhang, Wen-Qi Liu, Yong-Wen He
Hui Chen, Yong-Wen He,Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Jing-Hui Zhang,Department of Surgical Laboratory, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Wen-Qi Liu,Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported bythe Natural Science Foundation of Hubei Province, No. 2005ABA170
Correspondence to:Hui Chen, Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. chenhui0515@yahoo.com.cn
Received:2006-11-20 Accepted:2006-12-27
Abstract
AIM: To investigate the effect of rosiglitazone on the binding activity of hepatic nuclear factor-kappa B (NF-κB) and the expression of peroxisome proliferator-activated receptor gamma (PPARγ) in mice with liver fibrosis caused by schistosoma japonicum infection.
METHODS: A total of 50 mice were randomly and averagely divided into group A, B, C, D and E. The mice in group A served as normal controls, while those in the other four groups were infected with schistosoma japonicum to induce the model of liver fibrosis. Besides, the mice in group C, D and E were treated with praziquantel, rosiglitazone, and praziquantel plus rosiglitazone, respectively. HE staining was used to observe the pathological changes of liver tissues under light microscope, and Western blot and real-time fluorescent quantitative polymerase chain reaction (RFQ-PCR) were performed to detect the activity of NF-κB and mRNA expression of PPARγ.
RESULTS: The inflammatory and fibrotic degrees were obviously alleviated in group E, which were the lightest among those groups with schistosomiasis (P < 0.05). The activity of NF-κB was the highest in group B (141.11 ± 15.37), significantly higher than that in group A, C, D and E (78.89 ± 18.12, 112.89 ± 20.17, 108.89 ± 20.47, 88.89 ± 19.34)(P < 0.05). The level of PPARγ mRNA was markedly higher in group A [-16.557±(-3.022)], D [-18.217±(-4.498)] and E [-18.212±(-3.909)] than that in group B [-27 ......
谌辉, 贺永文, 华中科技大学同济医学院附属协和医院传染科 湖北省武汉市 430030
张景辉, 华中科技大学同济医学院附属协和医院外科实验室 湖北省武汉市 430030
刘文琪, 华中科技大学同济医学院寄生虫学教研室 湖北省武汉市 430022
湖北省自然科学基金资助项目, No. 2005ABA170
通讯作者: 谌辉, 430030, 湖北省武汉市, 华中科技大学同济医学院附属协和医院传染科. chenhui0515@yahoo.com.cn
电话: 027-85726419
收稿日期: 2006-11-20 接受日期: 2006-12-27
Effect of rosiglitazone on the activity of hepatic nuclear factor-kappa B and expression of peroxisome proliferator-activated receptor-γ in mice with liver fibrosis due to schistosoma japonicum infection
Hui Chen, Jing-Hui Zhang, Wen-Qi Liu, Yong-Wen He
Hui Chen, Yong-Wen He,Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Jing-Hui Zhang,Department of Surgical Laboratory, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Wen-Qi Liu,Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Supported bythe Natural Science Foundation of Hubei Province, No. 2005ABA170
Correspondence to:Hui Chen, Department of Infectious Diseases, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China. chenhui0515@yahoo.com.cn
Received:2006-11-20 Accepted:2006-12-27
Abstract
AIM: To investigate the effect of rosiglitazone on the binding activity of hepatic nuclear factor-kappa B (NF-κB) and the expression of peroxisome proliferator-activated receptor gamma (PPARγ) in mice with liver fibrosis caused by schistosoma japonicum infection.
METHODS: A total of 50 mice were randomly and averagely divided into group A, B, C, D and E. The mice in group A served as normal controls, while those in the other four groups were infected with schistosoma japonicum to induce the model of liver fibrosis. Besides, the mice in group C, D and E were treated with praziquantel, rosiglitazone, and praziquantel plus rosiglitazone, respectively. HE staining was used to observe the pathological changes of liver tissues under light microscope, and Western blot and real-time fluorescent quantitative polymerase chain reaction (RFQ-PCR) were performed to detect the activity of NF-κB and mRNA expression of PPARγ.
RESULTS: The inflammatory and fibrotic degrees were obviously alleviated in group E, which were the lightest among those groups with schistosomiasis (P < 0.05). The activity of NF-κB was the highest in group B (141.11 ± 15.37), significantly higher than that in group A, C, D and E (78.89 ± 18.12, 112.89 ± 20.17, 108.89 ± 20.47, 88.89 ± 19.34)(P < 0.05). The level of PPARγ mRNA was markedly higher in group A [-16.557±(-3.022)], D [-18.217±(-4.498)] and E [-18.212±(-3.909)] than that in group B [-27 ......
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