华夏小葱制剂对脂肪肝大鼠的防治作用
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常 青, 赵立波, 郝建军, 邴飞虹, 张介眉
华夏小葱制剂;脂肪肝;内皮素-1;肿瘤坏死因子-α;核转录因子-kB,常青,邴飞虹,赵立波,郝建军,张介眉,常青,通讯作者:,PreparationofChineseAlliumfistulosumLinninthepreventionandtre
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常青, 邴飞虹, 湖北中医学院中药研究所 湖北省武汉市 430061
赵立波, 华中科技大学同济医学院药理系 湖北省武汉市 430030
郝建军, 张介眉, 武汉市中西医结合医院 湖北省武汉市 430022
常青, 湖北中医学院硕士研究生, 主要从事药理学及中药化学研究.
湖北省卫生科研基金项目(鄂卫发), No. [2006]63号
通讯作者: 张介眉, 430022, 湖北省武汉市中山大道215号, 武汉市中西医结合医院院长办公室. zhangjiemei88@126.com
电话: 027-85332516 传真: 027-85332516
收稿日期: 2007-01-14 接受日期: 2007-01-20
Preparation of Chinese Allium fistulosum Linn in the prevention and treatment of rats with fatty liver disease
Qing Chang, Li-Bo Zhao, Jian-Jun Hao, Fei-Hong Bing, Jie-Mei Zhang
Qing Chang, Fei-Hong Bing,Institute of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China
Li-Bo Zhao,Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Jian-Jun Hao, Jie-Mei Zhang,Wuhan Hospital of Integrated Traditional Chinese and Western Medicine, Wuhan 430022, Hubei Province, China
Supported bythe Research Foundation of Public Health of Hubei Province, No. [2006]63
Correspondence to:Jie-Mei Zhang, Wuhan Hospital of Combination of Traditional Chinese Medicine with Western Medicine, 215 Zhongshan Road, Jiangan District, Hubei 430022, China. zhangjiemei88@126.com
Received:2007-01-14 Accepted: 2007-01-20
Abstract
AIM: To investigate the preventive and therapeutic effect of preparation of Chinese Allium fistulosum Linn (PCAL) on the rats with fatty liver disease (FLD) and its related mechanism.
METHODS: Sixty Sprague Dawley rats were randomly and averagely divided into group A, B, C, D, E and F. High-fat diet, ethanol, and carbon tetrachloride (CCl4) were used to induce the model of FLD in all the rats except those in group A, and the rats in group A served as blank controls. Then the rats in group C, D, E and F were treated with low-, middle-, and high-dose PCAL (0.06, 0.12, 0.24 g/kg, repectively), and Dongbao Gantai (DBGT, 0.7 g/kg), respectively. All the animals were sacrificed after 8 weeks. Liver index (liver/body weight ratio) was calculated, and the levels of plasma total cholesterol (TC) and triglyceride (TG) were determined by automatic biochemical analyzer. Plasma endothelin-1 (ET-1) was assayed by radioimmunoassay. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to measure the expression of tumor necrosis factor-α (TNF-α) mRNA and nuclear transcription factor-kB (NF-kB) in liver tissues, respectively.
RESULTS: Liver index, the levels of plasma TC, TG, ET-1, the expression of TNF-α mRNA and NF-kB p65 protein were significantly higher in group B that those in group A (3.45% ± 0.20% vs 2.33% ± 0.08%; 2.03 ± 0.38 mmol/L vs 1.39 ± 0.31 mmol/L; 1.18 ± 0.57 mmol/L vs 0.48 ± 0.08 mmol/L; 180.22 ± 9.80 ng/L vs 138.67 ± 21.84 ng/L; 0.84 ± 0.04 vs 0 ......
常青, 邴飞虹, 湖北中医学院中药研究所 湖北省武汉市 430061
赵立波, 华中科技大学同济医学院药理系 湖北省武汉市 430030
郝建军, 张介眉, 武汉市中西医结合医院 湖北省武汉市 430022
常青, 湖北中医学院硕士研究生, 主要从事药理学及中药化学研究.
湖北省卫生科研基金项目(鄂卫发), No. [2006]63号
通讯作者: 张介眉, 430022, 湖北省武汉市中山大道215号, 武汉市中西医结合医院院长办公室. zhangjiemei88@126.com
电话: 027-85332516 传真: 027-85332516
收稿日期: 2007-01-14 接受日期: 2007-01-20
Preparation of Chinese Allium fistulosum Linn in the prevention and treatment of rats with fatty liver disease
Qing Chang, Li-Bo Zhao, Jian-Jun Hao, Fei-Hong Bing, Jie-Mei Zhang
Qing Chang, Fei-Hong Bing,Institute of Traditional Chinese Medicine, Hubei College of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China
Li-Bo Zhao,Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Jian-Jun Hao, Jie-Mei Zhang,Wuhan Hospital of Integrated Traditional Chinese and Western Medicine, Wuhan 430022, Hubei Province, China
Supported bythe Research Foundation of Public Health of Hubei Province, No. [2006]63
Correspondence to:Jie-Mei Zhang, Wuhan Hospital of Combination of Traditional Chinese Medicine with Western Medicine, 215 Zhongshan Road, Jiangan District, Hubei 430022, China. zhangjiemei88@126.com
Received:2007-01-14 Accepted: 2007-01-20
Abstract
AIM: To investigate the preventive and therapeutic effect of preparation of Chinese Allium fistulosum Linn (PCAL) on the rats with fatty liver disease (FLD) and its related mechanism.
METHODS: Sixty Sprague Dawley rats were randomly and averagely divided into group A, B, C, D, E and F. High-fat diet, ethanol, and carbon tetrachloride (CCl4) were used to induce the model of FLD in all the rats except those in group A, and the rats in group A served as blank controls. Then the rats in group C, D, E and F were treated with low-, middle-, and high-dose PCAL (0.06, 0.12, 0.24 g/kg, repectively), and Dongbao Gantai (DBGT, 0.7 g/kg), respectively. All the animals were sacrificed after 8 weeks. Liver index (liver/body weight ratio) was calculated, and the levels of plasma total cholesterol (TC) and triglyceride (TG) were determined by automatic biochemical analyzer. Plasma endothelin-1 (ET-1) was assayed by radioimmunoassay. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to measure the expression of tumor necrosis factor-α (TNF-α) mRNA and nuclear transcription factor-kB (NF-kB) in liver tissues, respectively.
RESULTS: Liver index, the levels of plasma TC, TG, ET-1, the expression of TNF-α mRNA and NF-kB p65 protein were significantly higher in group B that those in group A (3.45% ± 0.20% vs 2.33% ± 0.08%; 2.03 ± 0.38 mmol/L vs 1.39 ± 0.31 mmol/L; 1.18 ± 0.57 mmol/L vs 0.48 ± 0.08 mmol/L; 180.22 ± 9.80 ng/L vs 138.67 ± 21.84 ng/L; 0.84 ± 0.04 vs 0 ......
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