Protein changes in immature dendritic cells infected with sendai virus vector

    YI Faª²Ping, ZHANG Pingª²Bo, GUO Fengª²Jin, MA Yongª²Ping, SONG Fangª²Zhou

    Department of Biochemistry and Molecular Biology, Chongqing University of Medical Sciences, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400016, China, Institute of Genetic Resources, Kyushu University, Fukuoka 812ª²8581, Japan

    ¡¾Abstract¡¿ AIM£º To investigate the protein changes in immature dendritic cells (DC) infected with sendai virus vector. METHODS: The total cellular proteins were collected and determined in the immature DC infected with sendai virus vector and the control ones uninfected with it. They were separated using 2ª²dimensional gel electrophoresis (2ª²DE) and visualized by silver staining. Digital images were analyzed by PDQuest software. The differentially expressed protein spots were picked and digested in gel, followed by identification with peptide mass fingerprinting (PMF) using MALDIª²TOF MS. RESULTS: More than 40 protein spots disappeared in 2ª²DE of the treated group. Among them 5 protein spots that were apparently not expressed were identified by PMF. CONCLUSION: Protein expression decreased in DC infected with sendai virus vector. ......