Establishment of a cell line stably expressing human cardiac Kv1.5 potasª²sium channel

    ZHAO Zhiª²Jing, WANG Xiaoª²Bin, MU Runª²Hua, LIU Yi, SUN Dongª²Dong, LI Zhiª²Chao, WANG Haiª²Chang, DONG Mingª²Qing

    Department of Cardiovasology, 3Department of Gynecology and Obstetrics, Xijing Hospital, Department of Pathophysiology, School of Basic Medicine, Fourth Military Medical University, Xi¬ðan 710033, China

    ¡¾Abstract¡¿ AIM: To establish a cell line stably expressing human cardiac Kv1.5 potassium channel. METHODS: Human cardiac hKv1.5 gene was transfected into HEK 293 cells. G418 at 1 mg/mL was used to select the transfected cells. After 2 weeks for selection, the antiª²G418 cell clones were further identified with patchª²clamp method, and whole cell currents were recorded. RESULTS: hKv1.5 gene was successfully transfected into HEK 293 cells. The expressed hKv1.5 currents were ultraª²rapid outward currents with obvious voltageª²dependence. Its average amplitude was (3.20¡À0.60) nA and its activation voltage was about -30 mV. CONCLUSION: The cell line stably expressing human atrial Kv1.5 channels has been successfully established, which has the similar characteristics of human atrial IKur. The established cell line can be used as a cell model for studying in vitro human atrial IKur. ......