小檗碱对胰岛素抵抗大鼠肝脏葡萄糖激酶及其调节蛋白的影响
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欧阳礼枝, 陆付耳, 刘文军, 高志强, 徐丽君
胰岛素抵抗;小檗碱;葡萄糖激酶;葡萄糖激酶调节蛋白,欧阳礼枝,陆付耳,刘文军,高志强,徐丽君,通讯作者:,电话:, 传真:,收稿日期:,接受日期:,Effectofberberineonhepaticglucokinase
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欧阳礼枝, 陆付耳, 刘文军, 高志强, 徐丽君, 华中科技大学同济医学院附属同济医院中西医结合研究所 湖北省武汉市 430030国家自然科学基金资助项目, No. 30472254
通讯作者: 陆付耳, 430030, 湖北省武汉市, 湖北省武汉市同济医院中西医结合研究所. felu@tjh.tjmu.edu.cn
电话: 027-83662577 传真: 027-83662220
收稿日期: 2006-12-07 接受日期: 2006-12-28
Effect of berberine on hepatic glucokinase and its regulatory protein in insulin-resistant rats
Li-Zhi Ouyang, Fu-Er Lu, Wen-Jun Liu, Zhi-Qiang Gao, Li-Jun Xu
Li-Zhi Ouyang, Fu-Er Lu, Wen-Jun Liu, Zhi-Qiang Gao, Li-Jun Xu,Institute of Integrated Chinese and Western Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported byNational Natural Science Foundation of China, No.30472254
Correspondence to:Fu-Er Lu, Institute of Integrated Chinese and Western Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province,China. felu@tjh.tjmu.edu.cn
Received:2006-12-07 Accepted:2006-12-28
Abstract
AIM:To investigate the effects of berberine on hepatic glucokinase (GK) activity, protein expression and GK’s regulatory protein (GKRP) in insulin-resistant rats.
METHODS: Male Wistar rats were fed with high-fat diet for 8 weeks to induce the model of insulin resistance. Then the insulin-resistant rats were randomly divided into 3 groups: model, berberine, and metformin groups which were treated with the corresponding preparations. Meanwhile, a normal control group was designed. The differences in insulin sensitivity, hepatic glycogen, hepatic GK activity, and protein expression (using Western blot) of hepatic GK and GKRP were compared among the groups.
RESULTS: As compared with those in model group, the insulin sensitivity (-4.93 ± 0.30 vs -5.35 ± 0.40, P < 0.05), hepatic glycogen level (136.58 ± 52.57 µg/g vs 65.88 ± 27.80 µg/g, P < 0.05), GK activity (226.55 ± 10.62 µkat/g vs 92.69 ± 6.43 µkat/g, P < 0.05), and hepatic GK protein expression (1.71±0.49 vs 1.24±0.22, P < 0.05) were significantly elevated in berberine group, while the expression of GKRP protein weaker (1.19 ± 0.20 vs 1.94 ± 0.56, P < 0.01). Meanwhile, the hepatic glycogen level in berberine group was markedly higher than that in metformin group (136.584 ± 52.574 µg/g vs 89.427 ± 31.971 µg/g, P < 0.05), but the other indexes were not significantly different between them (all P > 0.05).
CONCLUSION: Berberine may decrease insulin resistance by increasing of the hepatic GK activity.
Key Words: Insulin resistance; Berberine; Glucokinase; Glucokinase regulatory protein
Ou-Yang LZ, Lu FE, Liu WJ, Gao ZQ, Xu LJ. Effect of berberine on hepatic glucokinase and its regulatory protein in insulin-resistant rats. Shijie Huaren Xiaohua Zazhi 2007;15(8):885-889
摘要 ......
欧阳礼枝, 陆付耳, 刘文军, 高志强, 徐丽君, 华中科技大学同济医学院附属同济医院中西医结合研究所 湖北省武汉市 430030国家自然科学基金资助项目, No. 30472254
通讯作者: 陆付耳, 430030, 湖北省武汉市, 湖北省武汉市同济医院中西医结合研究所. felu@tjh.tjmu.edu.cn
电话: 027-83662577 传真: 027-83662220
收稿日期: 2006-12-07 接受日期: 2006-12-28
Effect of berberine on hepatic glucokinase and its regulatory protein in insulin-resistant rats
Li-Zhi Ouyang, Fu-Er Lu, Wen-Jun Liu, Zhi-Qiang Gao, Li-Jun Xu
Li-Zhi Ouyang, Fu-Er Lu, Wen-Jun Liu, Zhi-Qiang Gao, Li-Jun Xu,Institute of Integrated Chinese and Western Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China
Supported byNational Natural Science Foundation of China, No.30472254
Correspondence to:Fu-Er Lu, Institute of Integrated Chinese and Western Medicine, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province,China. felu@tjh.tjmu.edu.cn
Received:2006-12-07 Accepted:2006-12-28
Abstract
AIM:To investigate the effects of berberine on hepatic glucokinase (GK) activity, protein expression and GK’s regulatory protein (GKRP) in insulin-resistant rats.
METHODS: Male Wistar rats were fed with high-fat diet for 8 weeks to induce the model of insulin resistance. Then the insulin-resistant rats were randomly divided into 3 groups: model, berberine, and metformin groups which were treated with the corresponding preparations. Meanwhile, a normal control group was designed. The differences in insulin sensitivity, hepatic glycogen, hepatic GK activity, and protein expression (using Western blot) of hepatic GK and GKRP were compared among the groups.
RESULTS: As compared with those in model group, the insulin sensitivity (-4.93 ± 0.30 vs -5.35 ± 0.40, P < 0.05), hepatic glycogen level (136.58 ± 52.57 µg/g vs 65.88 ± 27.80 µg/g, P < 0.05), GK activity (226.55 ± 10.62 µkat/g vs 92.69 ± 6.43 µkat/g, P < 0.05), and hepatic GK protein expression (1.71±0.49 vs 1.24±0.22, P < 0.05) were significantly elevated in berberine group, while the expression of GKRP protein weaker (1.19 ± 0.20 vs 1.94 ± 0.56, P < 0.01). Meanwhile, the hepatic glycogen level in berberine group was markedly higher than that in metformin group (136.584 ± 52.574 µg/g vs 89.427 ± 31.971 µg/g, P < 0.05), but the other indexes were not significantly different between them (all P > 0.05).
CONCLUSION: Berberine may decrease insulin resistance by increasing of the hepatic GK activity.
Key Words: Insulin resistance; Berberine; Glucokinase; Glucokinase regulatory protein
Ou-Yang LZ, Lu FE, Liu WJ, Gao ZQ, Xu LJ. Effect of berberine on hepatic glucokinase and its regulatory protein in insulin-resistant rats. Shijie Huaren Xiaohua Zazhi 2007;15(8):885-889
摘要 ......
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