间充质干细胞；TCR_Vβ基因；移植物抗宿主病；脂肪

脂肪源间充质干细胞治疗aGVHD分子机制的初步研究

http://www.100md.com 《实用医技杂志》 2006年第17期

间充质干细胞；TCR,Vβ基因；移植物抗宿主病；脂肪,,],间充质干细胞；TCR,Vβ基因；移植物抗宿主病；脂肪,1材料与方法,2结果,3讨论,参考文献：

     [摘要] 目的：初步探讨成人脂肪源间充质干细胞(adult adipose tissuederived mesenchymal stem cells,AMSC)治疗急性移植物抗宿主病(acute graftversushost disease，aGVHD)的分子机制。方法：3例行异基因造血干细胞移植术后发生 aGVHD患者，以每公斤体重2×106个细胞剂量静脉输注AMSC；应用RTPCR扩增患者AMSC使用前后外周血单个核细胞的TCR Vβ24个亚家族的CDR3，了解患者TCR VβT细胞的分布情况。结果：患者发生aGVHD时，有TCR Vβ3及其他亚家族基因表达，输注AMSC后，GVHD得以有效控制，Vβ3不表达；当患者GVHD复发时，Vβ3基因又表达，治疗后不表达。结论：AMSC治疗aGVHD的分子机制可能与其抑制TCR Vβ亚家族基因表达有关，TCR Vβ3可能是AMSC作用的靶基因。

    [关键词] 间充质干细胞；TCR Vβ基因；移植物抗宿主病；脂肪

    Study on the Molecular Mechanism of Treatment with Adult Adipose Tissue Derived Mesenchymal Stem Cells on Acute GraftVersusHost Disease

    FANG Baijun,SONG Yongping,LIN Quande,et al

    (He'nan Tumor Hospital,He'nan Institute of Hematology,Zhengzhou 45000，China)

    Abstract:Objective To investigate the molecular mechanism of treatment with adult adipose tissuederived mesenchymal stem cells (AMSC) on acute graftversushost disease (aGVHD)patients.Methods A dose of 2×106 AMSC per kg of the aGVHD patients' weight was given intravenously.The distribution of TCR VβT repertoire was determined with amplification of the CDR3 of TCR Vβ 24 subfamily genes by using RTPCR in 3 cases with GVHD following allogeneic hematopoietic stem cell transplantation.Results It was found that they were expressions of TCR Vβ3 gene and other subfamily genes when GVHD was obvious,but they were not detected after AMSC treatment with remission of GVHD,while during relapse of the GVHD,expressions of Vβ3 gene reappeared,but disappeared after treatment.Conclusion The molecular mechanism for the treatment with AMSC on GVHD might be correlated with the inhibition of TCR Vβ subfamily gene expressions and the TCR Vβ3 gene might be the target gene for AMSC. ......

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