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正交试验优选—大孔树脂分离连翘酯苷的工艺
http://www.100md.com 《中华医学实践杂志》 2007年第1期
连翘;提取工艺;正交试验,,连翘;提取工艺;正交试验,1仪器与试药,2方法与结果,3讨论,【参考文献】
     正交试验优选—大孔树脂分离连翘酯苷的工艺 (pdf)

    【摘要】 目的 建立连翘中连翘酯苷的最佳提取和分离工艺。方法 确定提取溶剂后,以正交试验法考察加醇量、回流时间、回流次数、醇浓度4个因素及大孔树脂分离技术对连翘酯苷收率的影响。结果 连翘的最佳提取工艺为:加7倍量70%乙醇,回流提取3次,每次1.5h,大孔树脂洗脱液为40%乙醇。 结论 该工艺连翘酯苷收率为9.1%~10.6%(以药材计),连翘酯苷的含量为18.3%~22.5%,适合工业化生产。

    【关键词】 连翘;提取工艺;正交试验

    Extraction technology of forsythiaside from fructus forsythiae by the method of orthogonal test optimization and separation through macroporous resin

    GAO Xue-min, WANG Dong,JIN Xin,et al.Department of Pharmacy, Medical College, Xiamen University, Xiamen 361005, China.

    【Abstract】 Objective To design the extraction and separation technologcial conditions of forsythiaside from Fructus Forsythiae. Methods The solvent of extraction was investigated. The orthogonal test method was adopted to investigate the effect of the 4 factors, which include the concentration and volumn of alcohol, the time and the times of extraction and the process of absorption separation through macroporous resin column on the yielding rate of forsythiaside from Fructus Forsythiae. Results The optimum extraction condition were: extracting 3 times with 70% alcohol, 1.5 h each time with 7 times the volume of the alcohol. The eluent was 40% alcohol. Conclusion The results indicate that the yielding rate of forsythiaside from Fructus Forsythiae was from 9.1% to 10.6% by this technology. The concontration of forsythiaside in extract was from 18% to 22%, so this technology is suitable for industrial production. ......

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