青蒿素对小鼠Lewis肺癌移植瘤生长和淋巴管生成的影响
青蒿素;癌,Lewis肺;淋巴管生成;血管内皮生长因子C,,青蒿素;癌,Lewis肺;淋巴管生成;血管内皮生长因子C,【关键词】青蒿素;癌,Lewis肺;淋巴管生成;血管内皮生长因子C,0引言,1材料和方法,2结果,3讨论,【参考文
Effect of artemisinin on metastatic tumor growth and lymphangiogenesis in mice bearing Lewis lung carcinomaGUO Yan1, WANG Jun1,ZHANG BiCheng1, CHEN ZhengTang1, GAO JianFei2, ZHAO Yong2
1Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China, 2Department of Oncology, Wuhan General Hospital, Guangzhou Military Area Command, Wuhan 430070, China
【Abstract】 AIM: To explore the effect of antimalarial artemisinin on Lewis lung carcimoma (LLC) growth and tumor lymphangiogenesis. METHODS: The models of LLC in C57BL/6 mice were established via subcutaneous injection of mouse LLC cells. After mice were orally administered with artemisinin at 50 mg/kg body weight, once 2 d for 2 weeks, tumors were measured with calipers twice weekly. Mice were sacrificed 30 d following tumor cell injection, and Lewis tumors and lungs were harvested and weighted. Lewis tumors and lungs were processed for immunohistochemical analysis for lymphangiogenesis using VEGFR3 and LYVE1 staining or for micrometastasis using HE staining. Lymphatic microvessel density (LMVD) was evaluated by LYVE1 positive lymphatic vessel count. Furthermore, mice were monitored for survival studies. RESULTS: LMVD, wet weight of lungs and number of lung metastasis in artemisinintreated group were significantly lower as compared with control group (P<0.05). However there was not statistically significant difference in tumor growth between artemisinintreated mice and control mice (P>0.05). In addition, artemisinintreated mice lived significantly longer than the control mice did (P<0.01). CONCLUSION: Antimalarial artemisinin effectively inhibits the lymphangiogenesis and lung metastasis, and enhances the survival of mice bearing LLC. ......
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