紫外分光光度计法；湿重法；平板菌落计数法；标准曲线；牡牛分枝

分枝杆菌计数方法的比较研究

http://www.100md.com 《第四军医大学学报》 2007年第4期

紫外分光光度计法；湿重法；平板菌落计数法；标准曲线；牡牛分枝杆菌,,紫外分光光度计法；湿重法；平板菌落计数法；标准曲线；牡牛分枝杆菌,【关键词】紫外分光光度计法；湿重法；平板菌落计数法；标准曲线；牡牛分枝杆菌,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Comparative study of Mycobacterium counting methods

    LUO TaiLai1, SHEN LiangLiang1, BAI YinLan2, XUE Ying2

    1Team 1, Department of Pharmacy, 2Department of Microbiology, School of Basic Medicine, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To build up a fast counting method for Mycobacterium vaccae (M. vaccae). METHODS: We counted M. vaccae by UV spectrophotometry, wet weight measurement and plate count method, and built up two standard curves. One was drawn according to the results of UV spectrophotometry to plate count and the other was humid weight to plate count. RESULTS: We built up two standard curves with the results of UV spectrophotometry and wet weight, to plate count, respectively. The analysis of accuracy rating proved that there was an approximal linear correlation between the two standard curves ranging from 105/mL to 108/mL. CONCLUSION: The method is dependable, and can be used for quick counting of M. vaccae.

    【Keywords】 UV spectrophotometry; wet weight measurement; plate count; standard curve; Mycobacterium vaccae ......

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