灯盏花提取成分对体外高压培养视网膜神经节细胞凋亡基因表达的影响
视网膜神经节细胞；细胞培养；压力；细胞凋亡,,],视网膜神经节细胞；细胞培养；压力；细胞凋亡,1材料与方法,2结果,3讨论,[参考文献]
灯盏花提取成分对体外高压培养视网膜神经节细胞凋亡基因表达的影响 (pdf)[摘要] 目的 观察灯盏花提取物(DSX)对体外高压培养视网膜神经节细胞凋亡及凋亡基因表达的影响。 方法 (1)在RGCs中加入DSX，加压培养后收集细胞，用流式细胞仪测定凋亡细胞百分率。(2)在RGCs中加入DSX，加压培养后收集细胞，用基因芯片寻找凋亡基因的表达。结果 (1)在RGCs高压培养时，DSX组的凋亡百分率低于模型组和对照组。(2)在高压培养的条件下，与神经元细胞凋亡相关的基因S100B表达恢复正常，NGFR表达下调。 结论 DSX是具有RGCs保护作用的有效单体，能使高压培养RGCs的S100B的表达恢复正常，NGFR的表达下调。
[关键词] 视网膜神经节细胞；细胞培养；压力；细胞凋亡
Apoptotic genetic expression of RGCs by bolting constituent from Herba Erigerontis cultured in vitro by high pressure
ZENG Jieping，DUAN Junguo.Eye Lab of Chengdu University of TCM，Chengdu 610075，China
[Abstract] Objective To observe the apoptosis and look for apoptotic gene of RGCs cultured in vitro by high pressure by adding the constituent come from Herba Erigerontis(DSX). Methods We build rat retinal cells and RGCs cultured in virto by high pressure.Put 80 mmHg pressure to RGCs in close tightly culturebottle for four hours， then collect RGCs and check the sum of apoptosis cells by flow cytometry(FCM).Put the pressure 80 mmHg to RGCs in close tightly culturebottle for four hours ......
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