鼻黏膜耐受;_MBP6886;

经鼻黏膜给予MBP6886和8799协同免疫预防Lewis大鼠EAE的实验研究

http://www.100md.com 《细胞与分子免疫学杂志》 2007年第2期

鼻黏膜耐受;,MBP6886;,MBP8799;,实验性自身免疫性脑脊髓炎,,]鼻黏膜耐受;,MBP6886;,MBP8799;,实验性自身免疫性脑脊髓炎,1材料和方法,2结果,3讨论,参考文献:

     Inhibiton of experimental autoimmune encephalomyelitis in Lewis rats by nasal administration of encephalitogenic MBP peptides:synergisticeffectsofMBP6886 and 8799

    SUN Bo, YANG Shuo, PENG Haisheng, QIAO Hui, CAO Jingyan, JIN Lianhong, LI Hulun*

    DepartmentofNeurobiology,ProvinceNeurobiologyKeyLaboratory,HarbinMedical University,Harbin 150086, China

    [Abstract] AIM: To explore the synergistic effect of MBP6886 and 8799, on the inhibition of experimental autoimmune encephalomyelitis (EAE) in Lewis rat by nasal administration. METHODS: Three different MBP peptides(MBP6886, 8799, and the nonencephalitogenic peptide 110128) were synthesized and administrated nasally to Lewis rat on day11, 10, 9, 8 and 7 prior to immunization with the guinea pig MBP (gpMBP) + CFA, which was used to induce EAE. The protective effect on Lewis rat from EAE by the MBP peptides was evaluated. RESULTS: Protection was achieved with the encephalitogenic peptides MBP6886 and 8799, MBP6886 being more potent, but not with MBP110128. Neither MBP6886 nor 8799 used alone conferred complete protection to gpMBPinduced EAE. In contrast, nasal administration of a mixture of MBP6886 and 8799 completely blocked gpMBPinduced EAE even at lower dosage than being used alone. Rats tolerized with MBP6886+8799 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFNγ ELISPOT assays. Rats tolerized with MBP6886+8799 also had abrogated MBPreactive IFNγ and TNFα mRNA expression in lymph node cells compared to rats receiving MBP110128 nasally, while similar low levels of MBPreactive TGFβ and IL4 mRNA expressing cells were observed in the two groups. CONCLUSION: Nasal administration of encephalitogenic MBP peptides can induce antigenspecific T cell tolerance and confer incomplete protection to gpMBPinduced EAE, and MBP 6886 and 8799 have synergistic effecs. Nonregulatory mechanisms are proposed to be responsible for tolerance development after nasal peptide administration. ......

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