成骨细胞；超氧化物歧化酶；膜流动性；β_β胡萝卜素

β，β胡萝卜素改善H2O2对成骨细胞损伤的影响

http://www.100md.com 《西安交通大学学报》 2007年第1期

成骨细胞；超氧化物歧化酶；膜流动性；β,β胡萝卜素,,成骨细胞；超氧化物歧化酶；膜流动性；β，β胡萝卜素,1材料与方法,2结果,3讨论,参考文献：

     摘要：目的研究β，β胡萝卜素增强成骨细胞抗自由基损伤的作用。方法用H2O2作用M3T3E1成骨细胞，建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、β，β胡萝卜素低剂量组(1×10-8mol/L)、β，β胡萝卜素中剂量组(1×10-7mol/L)和β，β胡萝卜素高剂量组(1×10-6mol/L)。测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase, SOD)活性、活性自由氧(reactive oxygen species, ROS)含量、脂质过氧化物(lipid oxygen, LPO)含量，同时利用荧光偏振法测定细胞膜流动性。结果模型组细胞活力、SOD活性、细胞膜流动性均比不同β，β胡萝卜素剂量组有显著降低(P<0.01)，而ROS、LPO含量均比不同β，β胡萝卜素剂量组有显著升高(P<0.01)。结论 H2O2摄入会导致成骨细胞的氧化损伤，而β，β胡萝卜素可以预防或降低此类损伤对细胞的影响。

    关键词：成骨细胞；超氧化物歧化酶；膜流动性；β，β胡萝卜素

    Effect of β, βcarotene in improving H2O2 induced damage of osteoblasts

    Pei Lingpeng, Dong Fuhui, Hui Bodi, Zhu Jia

    (Bone Research Center, China Academy of Chinese Medicine Science, Beijing 100700; College of Applied Art & Science of Beijing Union University, Beijing 100083; The Pharmacology Lab of Bone Research Center, China Academy of Chinese Medicine Science, Beijing 100700, China)

    ABSTRACT: Objective To investigate the effect of β, βcarotene in enhancing the function of antioxidative damage in osteoblasts. Methods M3T3E1 osteoblasts were randomly divided in five groups: control group, model group, and β, βcarotene group [lowdose (1×10-8mol/L), mediumdose (1×10-7mol/L), highdose (1×10-6mol/L)]. The activity of cells, superoxide dismutase (SOD), the content of reactive oxygen species (ROS), lipid oxygen (LPO), and membrane fluidity were tested and compared. Results Compared with those in β, βcarotene groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased (P<0.01). However, the content of ROS and LPO was significantly raised(P<0.01). Conclusion H2O2 can cause oxidative damage of M3T3E1 osteoblasts, but β, βcarotene can prevent and decrease its influence. ......

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