【摘 要】 目的 观测血管性痴呆小鼠海马神经元N-甲基-D-天门冬氨酸(NMDA)受体免疫组织化学变化特征，以探讨其在血管性痴呆发病中的作用。 方法 双侧颈总动脉线结，反复缺血—再灌注法制备模型，利用跳台试验和水迷宫试验观测其行为学改变，采用免疫组织化学技术观测小鼠海马神经元N-甲基-D-天门冬氨酸受体蛋白的表达变化。 结果 模型组小鼠学习、记忆成绩较假手术组明显降低(P<0.01)，其海马N-甲基-D-天门冬氨酸受体蛋白表达也明显下降(P<0.01)。 结论 血管性痴呆小鼠学习、记忆成绩下降与其海马N-甲基-D-天门冬氨酸受体蛋白低水平表达有关。

    【关键词】 痴呆 ，血管性;N-甲基-D-天门冬氨酸;免疫组织化学;小鼠

    The features of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia by immunohistochemistry ZHAI Jinping

    ，CHEN Li，LV Peiyuan，et al.

    * Department of Pathology，Hebei Provincial People's Hospital，Shijiazhuang 050051，China

    【Abstract】 Objective The characteristics of N-methyl-D-aspartate receptor in neuron of hippocampus of mice with vascular dementia(VD)were observed for the purpose of demonstrating its function in VD pathogenesis.Methods The mice were subjected for ischemia-reperfusion repeatedly in bilateral common carotid arteries by knot-ting to establish the VD models.The behavior abnormalities were investigated by step-down test and water maze test ......