淋巴细胞培养试验(研究原著)

siRNA干扰ADAR1表达对小鼠混合淋巴细胞培养的影响

http://www.100md.com 《第四军医大学学报》 2000年第5期

淋巴细胞培养试验,混合；RNA干扰；ADAR1基因；siRNA；排斥反应,,淋巴细胞培养试验，混合；RNA干扰；ADAR1基因；siRNA；排斥反应,【关键词】淋巴细胞培养试验，混合；RNA干扰；ADAR1基因；siRNA；排斥反应,0

     Effect of siRNAmediated silencing of ADAR1 gene on mixed mouse lymphocyte culture

    CAI Lei, HUO BinLiang, ZHANG Wei, ZHANG FuQin, ZHAO QingChuan

    Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xian 710033, China

    【Abstract】 AIM: To investigate lymphocyte proliferation when ADAR1 is suppressed in mixed lymphocyte culture test. METHODS: Primary lymphocyte was isolated from Balb/c and C57 mice. ADAR1 siRNA was transferred into the lymphocyte cell using Lipofectamine 2000 before twosource lymphocytes were mixed and cultured. The cell morphology was observed under a light microscope, the cell proliferation was checked using cell counting, trypan blue staining and the cell viability was determined by MTT assay. The ADAR1 mRNA was detected by RTPCR. RESULTS: When the ADAR1 expression was suppressed by the specific siRNA, the lymphocyte proliferation was inhibited in mixed lymphocyte culture test. CONCLUSION: ADAR1 has a potential role in the proliferation of mouse lymphocyte.

    【Keywords】 lymphocyte culture test, mixed; RNAi; ADAR1; siRNA; rejection ......

百拇医药网 http://www.100md.com/html/DirDu/2007/04/04/41/15/20.htm

您现在查看是摘要页，全文长 10994 字符。