Biological characteristics of monocyteª²derived dendritic cells from peripheral blood of patients with oral carcinoma

    ZHANG Suª²Xin, WANG Shiª²Jie, SHAN Baoª²En, DUAN Yuª²Qin, XU Yanª²Zhi

    Department of Stomatology, Fourth Hospital, Hebei Medical University, Shijiazhuang 050011, China

    ¡¾Abstract¡¿ AIM: To investigate the morphology, phenotype and functions of dendritic cell (DC) from peripheral blood of the healthy individuals and the patients with oral carcinoma. METHODS: Fifteen patients with oral carcinoma at stages of II or above were assigned to study group and 15 healthy volunteers to control group. Monocytes were isolated by density centrifugation, and cultured in the presence of GMª²CSF, ILª²4 and TNFª²¦Á for 7 d in vitro. The morphological change of the cultured cells was observed by light microscopy and electron microscopy. The expressions of CD83, CD1a, CD86, CD80, HLAª²DR were analyzed by flow cytometry. The ability of DC to stimulate the proliferation of lymphocyte in mixed lymphocyte reactions (MLR) was determined with the method of MTT. RESULTS: Morphologically and phenotypically typical DC was obtained in the 2 groups after culture for 7 d. The expression rates of CD83, CD1a on DC from the patients were 49.3¡À9.5 and 48.1¡À7.3 respectively ......