硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤
硫酸镍;睾丸;线粒体;微粒体;氧化应激,,硫酸镍;睾丸;线粒体;微粒体;氧化应激,1材料与方法,2结果,3讨论,参考文献
摘要: 目的 从氧化应激角度探讨硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性作用。方法 健康性成熟Wistar雄性大鼠32只,随机分为4组:生理盐水(NS)组,硫酸镍(NiSO4)125,250,500mg/kg组,等容积腹腔注射染毒,1次/d,连续30d。制备睾丸组织匀浆,离心提取线粒体和微粒体,采用分光光度法检测睾丸细胞线粒体及微粒体中脂质过氧化物(LPO)、总抗氧化能力(TAOC)、活性氧(ROS)水平的变化。结果 染毒组与对照组比较,大鼠睾丸脏器系数无明显变化(P>005)。镍可引起睾丸组织中LPO、ROS含量升高,与对照组比较差异有统计学意义(P<005);各染毒组TTOC均低于对照组(P<005)。结论 镍对大鼠睾丸细胞的损伤可能与其所致的睾丸细胞线粒体及微粒体氧化应激效应增强有关。关键词: 硫酸镍;睾丸;线粒体;微粒体;氧化应激
Oxidative damage of mitochondria and micriosome in testis cells induced by nickel sulfate in rats
SUN Yingbiao,CHEN Jianhua,LIU Yiya,et al.
School of Public Health,Lanzhou University(Lanzhou 730000,China)
Abstract: Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.Methods Thirtytwo male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraperitoneally with nickel sulfate daily at doses 125,250 ......
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