高效液相法；_慢支紫红丸；_岩白菜素____

高效液相法测定慢支紫红丸中岩白菜素的含量

http://www.100md.com 《时珍国医国药》 2007年第2期

高效液相法；,慢支紫红丸；,岩白菜素,,高效液相法；,慢支紫红丸；,岩白菜素,1仪器和试药,2方法与结果,3讨论

     摘要：目的建立了慢支紫红丸中岩白菜素的高效液相法(HPLC)测定方法。方法使用甲醇溶液加热回流提取岩白菜素，采用 RP18 C18柱为固定相，甲醇水(25∶75)为流动相，流速1 ml/min，检测波长275 nm。结果在0.053 5～0.535 μg范围内，岩白菜素与峰面积呈良好的线性关系，r＝0.999 9，平均加样回收率为98.2%,RSD=2.15%,n=9。结论该方法简便、快速、灵敏、专属性好,是制剂质量控制的有效方法。

    关键词：高效液相法；慢支紫红丸；岩白菜素

    Determination of Bergenin in Manzhizihong Pills by HPLC

    FENG Chong, HUANG Xiaodan, QIN ChunMei

    (Guangzhou Wanglaoji Pharmaceutical Company Limited， Guangzhou 510405,China)

    Abstract:ObjectiveAn HPLC method for determination of bergenin in Manzhizihong Pills was established.MethodsBergenin was extracted by methanol and warmup and reflux. An RP C18 column was used with the mobile phase of methanol-water(25∶75), at the detection wavelength of 275 nm and the flow rate was 1.0ml/min. ResultsThe linear range was 0.0535～0.535 and the average recovery was 98.2% (RSD 2.15%).ConclusionThe method is rapid,convenient.

    Key words:HPLC; Manzhizihong Pills; Bergenin

    慢支紫红丸由矮地茶、红背叶根、猪胆粉、五指毛桃和山药组成 ......

百拇医药网 http://www.100md.com/html/DirDu/2007/04/28/42/94/62.htm

您现在查看是摘要页，全文长 5042 字符。