高效液相色谱；_半边旗；_黄酮；_木犀草素_

高效液相色谱法测定半边旗中木犀草素的含量

http://www.100md.com 《时珍国医国药》 2007年第1期

高效液相色谱；,半边旗；,黄酮；,木犀草素,,高效液相色谱；,半边旗；,黄酮；,木犀草素,1仪器与试剂,2方法和结果,3讨论,参考文献：

     摘要：目的建立草药半边旗中木犀草素含量的准确测定方法，并测定不同采收期半边旗中木犀草素的含量。方法采用高效液相色谱法，色谱柱为Diamonsil C18，流动相为甲醇水梯度洗脱，流速0.2 ml·min1，检测波长为283 nm，柱温度35℃。结果木犀草素进样量在0.062~0.62 μg范围内线性关系良好(R2=0.998 7)，平均回收率为99.5%(RSD=1.98%)。结论该方法操作简便，结论可靠，可用于半边旗草药的质量控制。

    关键词：高效液相色谱；半边旗；黄酮；木犀草素

    Quantitative Determination of Luteolin in Pteris semipinnata L. by HPLC

    L Ying-nian, WU Kefeng, DENG Yifeng, LIANG Nianci

    (Guangdong Medical College, Guangdong Key Lab for Research and Development of Natural Drug, Zhanjiang 524023, China)

    Abstract：ObjectiveTo establish an HPLC method for content determination of luteolin from PSL.MethodsThe separation was performed on a Diamonsil C18 column, the mobile phase was run with a mixture of methanol(B) and water(A) with a linear gradient elution at a flow rate of 0.2 ml·min1, the ultraviolet wavelength was 283 nm, and the column temperature was maintained at 35℃. ResultsThe calibration curve showed good linearity in the range of 0.062~0.62 μg(R2=0.998 7), the average recovery was 99.5%(RSD=1.98%). ConclusionThe method is simple, convenient and accurate and can be used for the quality control of PSL. ......

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