灵芝；_富锗；_氨基酸；_超氧化物歧化酶____

富锗灵芝菌丝体氨基酸及超氧化物歧化酶含量的测定

http://www.100md.com 《时珍国医国药》 2005年第12期

灵芝；,富锗；,氨基酸；,超氧化物歧化酶,,灵芝；,富锗；,氨基酸；,超氧化物歧化酶,1材料与仪器,2方法,3实验结果与讨论,参考文献：

     摘要：目的：测定富锗灵芝菌丝体氨基酸及超氧化物歧化酶(SOD)的含量，探讨培养基中GeO2的最适加量。方法：高效液相色谱仪分析氨基酸，氯化硝基四氮唑蓝(NBT)光化还原法测定SOD的含量。结果：培养基中添加400 mg·L-1的GeO2，灵芝菌丝体中氨基酸含量比对照提高54.34%，SOD提高16.73%。结论：为富锗灵芝菌丝体的应用奠定基础。

    关键词：灵芝；富锗；氨基酸；超氧化物歧化酶

    Determination of SOD and Amino Acids in Geenriched Mycelia of Ganoderma lucidum

    DANG Jianzhang, SUN Haiyan, XU Baiqiu, FENG Lixiong

    (Department of Applied Biological Engineering, Shenzhen Polytechnic College, Shenzhen，Guangdong 518055, China)

    Abstract:Objective:To determine the contents of SOD and amino acids in Geenriched mycelia of Ganoderma lucidum and explore the optimum dosage of GeO2 in culture medium.Methods:The contents of amino acids were analyzed by high performance liquid chromatography and SOD was determined by nitroblue tetrazolium photoreduction method.Results:Adding GeO2 400 mg·L-1 to the culture medium the contents of amino acids and SOD in Geenriched mycelia of Ganoderma lucidum increase by 54.34% and 16.73% respectively over normal mycelia of Ganoderma lucidum.Conclusion:It provides a firm foundation for application of Geenriched mycelia of Ganoderma lucidum. ......

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