肺腺癌细胞对谷胱甘肽纳米Fe3O4的内吞作用
人肺腺癌细胞；谷胱甘肽；Fe3O4；内吞作用,,人肺腺癌细胞；谷胱甘肽；Fe3O4；内吞作用,1材料与方法,2结果,3讨论,参考文献
摘要： 目的 在体外观察人肺腺癌细胞(SPC-A1)对谷胱甘肽(GSSG)修饰的纳米Fe3O4的内吞作用及与培养温度、时间及浓度的关系。方法 通过透射电镜(TEM)与普鲁士兰染色法观察SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞作用。采用原子吸收光谱(AAS)法进行SPC-A1细胞粘附量及内吞量的定量分析。结果 TEM结果表明，在4℃下培养24h后GSSG修饰的纳米Fe3O4吸附于SPC-A1细胞表面；37℃下培养1h后SPC-A1细胞可内吞GSSG修饰的纳米Fe3O4细胞数目明显增加。AAS分析表明，SPC-A1细胞表面纳米Fe3O4的粘附量同培养时间及浓度呈正相关。GSSG修饰的纳米Fe3O4浓度较低(01或04mg/ml)时，内吞量在24h后达到饱和；GSSG修饰的纳米Fe3O4浓度较高(07或10mg/ml)时，内吞量在72h后达到饱和，单个细胞内吞量最大可达160pg。结论 SPC-A1细胞对GSSG修饰的纳米Fe3O4的内吞需要能量，内吞量依赖于浓度及培养时间。关键词： 人肺腺癌细胞；谷胱甘肽；Fe3O4；内吞作用
Endocytosis of lung adenocarcinoma cell on glutathione modified magnetite(Fe3O4)nanoparticles
YAN Zhubing,MA Yongjie,GU Hongchen,et al.
Institute of Micro and Nano Science and Technology,Shanghai Jiaotong University(Shanghai 200030,China)
Abstract： Objective To study the relationship between endocytosis of human lung adenocarcinoma cell line SPC-A1 modified by oxidized Glutathione(GSSG) and culture conditions including culture temperature,time and concentration.Methods The uptake of GSSG-modified Fe3O4 nanoparticles by SPC-A1 cell was observed by transmission electron microscopy (TEM) and Prussian blue staining.The extracellular adhesion or intracellular uptake of GSSG-modified Fe3O4 nanoparticles were measured by atom absorb spectrum (AAS).Results TEM results indicated that GSSG-modified Fe3O4 nanoparticles adhered on the membrane of SPC-A1 cell after being cultured at 4℃ for 1h or 24h,but SPC-Al cell could took up nanoparticles particles after cultured at 37℃ for 1h.Prussian blue staining indicated that SPC-Al cells containing GSSG-modified Fe3O4 nanoparticles were much more with the prolongation of culture time or the increase of concentration.AAS analysis indicated that the extracellular adhesion of GSSG-modified magnetic particles was depended on culture time and concentration.The uptake amount of GSSG-modiied Fe3O4 nanoparticles by SPC-A1 cell was saturated after 24h when the nanoparticles in low concentration (0.1mg/ml or 0.4mg/ml),the uptake was saturated after 72h when the nanoparticles in high concentration (0.7mg/ml or 1.0mg/ml).The maximal amount per SPC-A1 cell could reach 160pg.Conclusion The uptake of GSSG-modified Fe3O4 nanoparticles by SPC-A1 cell is energy needed.It depends on the concentration of nanoparticles and saturates at certain time. ......
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