癌_肝细胞；_mcl1；_RNA干扰_

siRNA对HepG2细胞mcl1表达的影响

http://www.100md.com 《第四军医大学学报》 2007年第6期

癌,肝细胞；,mcl1；,RNA干扰,,癌，肝细胞；,mcl1；,RNA干扰,【关键词】癌，肝细胞；mcl1；RNA干扰,0引言,1材料和方法,2结果,3讨论,【参考文献】

     Effect of siRNA on mcl1 expression in HepG2 cells

    ZHANG Wei1， ZHAO Pu2， CAO DaYong1，TAO KaiShan1， CAI Lei1， ZHANG FuQin1， DOU KeFeng1

    1Department of Hepatobiliary Surgery， Xijing Hospital， 2Department of Cell Biology， School of Basic Medicine, Fourth Military Medical University，Xian 710033， China

    【Abstract】 AIM: To investigate the effect of specific siRNA on mcl1 expression in hepatoma cell line HepG2. METHODS: Human hepatoma cell line HepG2 was cultured in vitro and transfected with two siRNAs(different loci) of mcl1 by lipofectamine 2000. The levels of mcl1 mRNA expression before and after transfection were detected by semiquantitative reverse transcription polymerase chain reaction(RTPCR).The interfering effect between the two siRNAs and the specificity and time effect for interference were compared.Then the levels of Mcl1 protein expression before and after transfection were detected by Western Blot. RESULTS： The level of mcl1 mRNA in HepG2 cells was inhibited by the specific siRNAs.The expression of mcl1 mRNA began to decrease 24 h after transfection; and the most apparent interfering efficiency was 67.25% 48 h after transfection ......

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