犬脑梗塞应激时蔗糖通透性检测评价胃屏障功能的研究
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犬脑梗塞应激时蔗糖通透性检测评价胃屏障功能的研究
刘业成 齐志伟 马遂
中国协和医科大学北京协和医院急诊科 100730
摘要：目的：评估脑梗塞应激时胃屏障功能变化，并检测用蔗糖通透性评价胃屏障的有效性。方法：20只犬参与实验。10只犬制做脑梗塞模型后，胃管入含蔗糖50g的NS300ml，检测5小时和24小时的蔗糖通透性，24h后取胃病理做大体分级和组织学积分，比较这些结果和伪手术组10只犬相应结果有无区别。同时把蔗糖通透性和大体病理及组织学积分结果做相关性分析，以判断蔗糖通透性检测的有效性。结果：实验组和伪手术组比较，5小时和24小时尿蔗糖通透性都有显著差异( p<0.05)，病理的大体分级和组织学积分都有显著性差异(p<0.05)；蔗糖通透性和胃大体病理结果程正相关，(r=0.89,p<0.05)，和胃组织学病理积分结果也程正相关(r=0.83,p<0.05)。结论：脑梗塞应激时胃屏障功能有显著破坏，蔗糖通透性检测可以作为评价胃屏障功能的可靠而易行的办法。
关键词： 应激，脑梗塞，胃屏障功能
The investigation of evaluating the function of gastric barrier by sucrose permeability in the stress of cerebral infarction in dogs
Department of emergency medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, china
Abstract: Objective: To evaluate the change of gastric barrier function in the stress of cerebral infarction, and assess the value of sucrose permeability in the evaluation of gastric barrier.Method:20 dogs were studied.10 dogs were made cerebral infarction by operation and fed with 50g sucrose within 300ml NS by gastric tube, and the later 5h and 24h urine sucrose excretion were measured. The dogs were sacrificed for gastric gross classification and histological score 24h after infarction. All the result were compared with the 10 dogs in sham group. Combined with the result of the gastric gross classification and histological score ,the result of the sucrose permeability were analyzed. Result :The sucrose permeability, gastric gross classification and histological score in the infarction group were all significantly different(p<0.05) from the sham group. And the result of the sucrose permeability were correlated with that of the gastric gross classification (r=0.89,p<0.05)and histological score(r=0.83,p<0.05).Conclusion: The gastric barrier were severe damaged in cerebral infarction, and the measurement of sucrose permeability is a simple and reliable means of assessing gastric barrier function. ......
刘业成 齐志伟 马遂
中国协和医科大学北京协和医院急诊科 100730
摘要：目的：评估脑梗塞应激时胃屏障功能变化，并检测用蔗糖通透性评价胃屏障的有效性。方法：20只犬参与实验。10只犬制做脑梗塞模型后，胃管入含蔗糖50g的NS300ml，检测5小时和24小时的蔗糖通透性，24h后取胃病理做大体分级和组织学积分，比较这些结果和伪手术组10只犬相应结果有无区别。同时把蔗糖通透性和大体病理及组织学积分结果做相关性分析，以判断蔗糖通透性检测的有效性。结果：实验组和伪手术组比较，5小时和24小时尿蔗糖通透性都有显著差异( p<0.05)，病理的大体分级和组织学积分都有显著性差异(p<0.05)；蔗糖通透性和胃大体病理结果程正相关，(r=0.89,p<0.05)，和胃组织学病理积分结果也程正相关(r=0.83,p<0.05)。结论：脑梗塞应激时胃屏障功能有显著破坏，蔗糖通透性检测可以作为评价胃屏障功能的可靠而易行的办法。
关键词： 应激，脑梗塞，胃屏障功能
The investigation of evaluating the function of gastric barrier by sucrose permeability in the stress of cerebral infarction in dogs
Department of emergency medicine, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, china
Abstract: Objective: To evaluate the change of gastric barrier function in the stress of cerebral infarction, and assess the value of sucrose permeability in the evaluation of gastric barrier.Method:20 dogs were studied.10 dogs were made cerebral infarction by operation and fed with 50g sucrose within 300ml NS by gastric tube, and the later 5h and 24h urine sucrose excretion were measured. The dogs were sacrificed for gastric gross classification and histological score 24h after infarction. All the result were compared with the 10 dogs in sham group. Combined with the result of the gastric gross classification and histological score ,the result of the sucrose permeability were analyzed. Result :The sucrose permeability, gastric gross classification and histological score in the infarction group were all significantly different(p<0.05) from the sham group. And the result of the sucrose permeability were correlated with that of the gastric gross classification (r=0.89,p<0.05)and histological score(r=0.83,p<0.05).Conclusion: The gastric barrier were severe damaged in cerebral infarction, and the measurement of sucrose permeability is a simple and reliable means of assessing gastric barrier function. ......