猫心肌缺血再灌注对Ca2+-ATPase活力及肌浆网钙摄取能力的影响
作者：陈 龙 胡寿鹏 张荣良 等
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摘要 目的摘要 目的: 观察猫心肌缺血再灌注过程中心肌细胞膜Ca2+-ATPase活力、肌浆网钙摄取能力、线粒体丙二醛及心肌ATP含量变化。方法: 利用猫体外循环模型,心脏低温停搏60 min后,恢复正常血液灌注60 min。测定心肌细胞膜Ca2+-ATPase活力、肌浆网钙摄取能力、心肌ATP及线粒体丙二醛含量。结果: 心脏缺血60 min时,随着心肌ATP含量的下降,Ca2+-ATPase活力及肌浆网钙摄取能力下降,而丙二醛含量仅有轻微增加;恢复灌注60 min后,Ca2+-ATPase活力及肌浆网钙摄取能力进一步下降,同时丙二醛含量明显增加。结论: 缺血期间心肌细胞膜Ca2+-ATPase活力及肌浆网钙摄取能力的下降主要与心肌能量储备降低有关,而复灌后Ca2+-ATPase活力及肌浆网钙摄取能力进一步下降,可能与复灌过程中氧自由基大量产生,使膜脂质过氧化有关。
中国图书资料分类法分类号 R654.2
Effects of ischemia/reperfusion injury on Ca2+-ATPase activity and calcium uptake of cardiac sarcoplasmic reticulum in cat heartChen Long, Hu Shoupeng, Zhang Rongliang, Wei Yaomei, Miao Mingyong (Department of Cardiothoracic Surgery, Fuzhou General Hospital, Nanjing Command, Fuzhou, 350001)
Abstract Objective: To observe the changes of myocardial Ca2+-ATPase activity and Ca2+ uptake during myocardial ischemia/reperfusion in cats. Methods: Twenty-four cats were divided into three groups. Group Ⅰ was not subjected to ischemia or reperfusion injury. Group Ⅱ was subjected to 60 min hypothermic ischemia. Group Ⅲ was subjected to 60 min hypothermic ischemia and 60 min reperfusion. Results: Myocardial ATP content was markedly decreased after 60 min ischemia. Meanwhile myocardial Ca2+-ATPase activity and Ca2+ uptake were markedly decreased. But Ca2+-ATPase activity and Ca2+ uptake were further decreased on the basis of ischemia. Malondialdehyde (MDA) content elevated apparently after reperfusion. Conclusion: The decreased activity of Ca2+-ATPase and Ca2+ uptake during ischemia may have a relation to the low myocardial ATP content, and the furthe>Transfer interrupted!, http://www.100md.com
单位：
关键词：
摘要 目的摘要 目的: 观察猫心肌缺血再灌注过程中心肌细胞膜Ca2+-ATPase活力、肌浆网钙摄取能力、线粒体丙二醛及心肌ATP含量变化。方法: 利用猫体外循环模型,心脏低温停搏60 min后,恢复正常血液灌注60 min。测定心肌细胞膜Ca2+-ATPase活力、肌浆网钙摄取能力、心肌ATP及线粒体丙二醛含量。结果: 心脏缺血60 min时,随着心肌ATP含量的下降,Ca2+-ATPase活力及肌浆网钙摄取能力下降,而丙二醛含量仅有轻微增加;恢复灌注60 min后,Ca2+-ATPase活力及肌浆网钙摄取能力进一步下降,同时丙二醛含量明显增加。结论: 缺血期间心肌细胞膜Ca2+-ATPase活力及肌浆网钙摄取能力的下降主要与心肌能量储备降低有关,而复灌后Ca2+-ATPase活力及肌浆网钙摄取能力进一步下降,可能与复灌过程中氧自由基大量产生,使膜脂质过氧化有关。
中国图书资料分类法分类号 R654.2
Effects of ischemia/reperfusion injury on Ca2+-ATPase activity and calcium uptake of cardiac sarcoplasmic reticulum in cat heartChen Long, Hu Shoupeng, Zhang Rongliang, Wei Yaomei, Miao Mingyong (Department of Cardiothoracic Surgery, Fuzhou General Hospital, Nanjing Command, Fuzhou, 350001)
Abstract Objective: To observe the changes of myocardial Ca2+-ATPase activity and Ca2+ uptake during myocardial ischemia/reperfusion in cats. Methods: Twenty-four cats were divided into three groups. Group Ⅰ was not subjected to ischemia or reperfusion injury. Group Ⅱ was subjected to 60 min hypothermic ischemia. Group Ⅲ was subjected to 60 min hypothermic ischemia and 60 min reperfusion. Results: Myocardial ATP content was markedly decreased after 60 min ischemia. Meanwhile myocardial Ca2+-ATPase activity and Ca2+ uptake were markedly decreased. But Ca2+-ATPase activity and Ca2+ uptake were further decreased on the basis of ischemia. Malondialdehyde (MDA) content elevated apparently after reperfusion. Conclusion: The decreased activity of Ca2+-ATPase and Ca2+ uptake during ischemia may have a relation to the low myocardial ATP content, and the furthe>Transfer interrupted!, http://www.100md.com