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任功一* 赵新泰 万大方 顾健人**
PCR|DI,TRISEC
作者：任功一* 赵新泰 万大方 顾健人**
单位：上海市肿瘤研究所，癌基因及相关基因国家重点实验室(上海200032)
关键词：PCR；DI/TRISEC
肿瘤990322 目的 PCR产物的克隆。 方法 通过DI/TRISEC法克隆PCR产物。 结果 PCR产物被高效定向地克隆。 结论 DI/TRISEC是一种方便和高效的PCR产物克隆方法。
A NEW PCR CLONING METHOD——DI/TRISEC
Ren Gongyi,Zhao Xintai,Wan Dafang,et al.National Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032
Objective：A new method of PCR product cloning is developed.Methods：DI/TRISEC.Results：PCR products was efficiently and directionally cloned into plasmid.Conclusion：DI/TRISEC is an efficient and convenient method for PCR product cloning.
Key words PCR Di- and tri-nucleotide sticky end cloning(DI/TRISEC)
PCR在分子生物学中应用非常广泛 ......
单位：上海市肿瘤研究所，癌基因及相关基因国家重点实验室(上海200032)
关键词：PCR；DI/TRISEC
肿瘤990322 目的 PCR产物的克隆。 方法 通过DI/TRISEC法克隆PCR产物。 结果 PCR产物被高效定向地克隆。 结论 DI/TRISEC是一种方便和高效的PCR产物克隆方法。
A NEW PCR CLONING METHOD——DI/TRISEC
Ren Gongyi,Zhao Xintai,Wan Dafang,et al.National Laboratory for Oncogenes and Related Genes,Shanghai Cancer Institute,Shanghai 200032
Objective：A new method of PCR product cloning is developed.Methods：DI/TRISEC.Results：PCR products was efficiently and directionally cloned into plasmid.Conclusion：DI/TRISEC is an efficient and convenient method for PCR product cloning.
Key words PCR Di- and tri-nucleotide sticky end cloning(DI/TRISEC)
PCR在分子生物学中应用非常广泛 ......