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p16基因在鼻咽癌中缺失和表达改变的研究——p16基因在鼻咽癌中的改变系列研究(Ⅰ)
http://www.100md.com 《癌症》1999年第4期
p16基因|表达下调|缺失
     作者:卓缨 曹亚

    单位:湖南医科大学肿瘤研究所分子生物室(长沙市,410078)

    关键词:p16基因;表达下调;缺失

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    【摘要】目的:探讨p16基因是否在鼻咽癌活检组织中存在高频的失活,以确定该基因在鼻咽癌发病过程中所起的作用。方法:首先利用免疫组化的方法检测了14例鼻咽癌活检组织中p16基因的表达状况。并用比较多重PCR-Southern杂交的方法检测了这14例鼻咽癌活检组织中p16基因外显子1α和外显子2可能的缺失,以了解导致p16基因表达下调的机制。结果:在14例鼻咽癌活检组织中有12例(~85%)缺乏p16蛋白的表达,但其中只有2例鼻咽癌活检组织存在外显子2的缺失。结论:以上的数据显示p16基因在鼻咽癌中存在高频率的失活,提示该基因在鼻咽癌发病过程中具有一定的作用,同时,上述结果也提示p16基因的缺失并不是鼻咽癌中造成p16基因表达下调的主要原因。

    中图分类号:R739.63R730.231; 文献标识码:A 文章编号:1000-467X(1999)04-0368-04

    Down-expression and deletion of the p16 gene in nasopharyngeal carcinoma: Serial research on alterations of the p16 gene in nasopharyngeal carcinoma (Ⅰ)

    ZHUO Ying,CAO Ya

    Cancer Research Institute of Hunan Medical University, Changsha 410078, P. R. China

    【Abstract】Objectives: To investigate whether inactivation of the p16 gene is frequent in nasopharyngeal carcinoma (NPC) biopsies, and to determine that the p16 gene takes part in nasapharyngeal carcinogenesis. Methods: In the present study, the expression of p16 gene in 14 NPC biopsies was detected by immunohistochemistry, and the possible deletion of p16 gene exon 1α and exon 2 was also detected in these 14 NPC biopsies by comparative multiplex PCR-Southern blotting to understand the mechanism of p16 gene down-regulation. Results:It was found that 12/14 (~85%) NPC biopsies had no detectable p16 protein, but only 2/14 (~14%) biopsies showed deletion in exon. 2. Conclusion: These data suggests that the p16 gene is frequently inactivated in NPC and it may play a role in NPC carcinogenesis. It also suggests that there may be some other genetic alterations besides deletion accounting for the p16 gene down-regulation. ......

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