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人脑肿瘤中SV40大T抗原与pRb形成特异性复合物
http://www.100md.com 《癌症》1999年第4期
脑肿瘤|SV40|大T抗原|抑癌蛋白|pRb
     作者:甄海宁 章翔 步星耀 黄文晋 张志文 肖华胜

    单位:第四军区大学 甄海宁 章翔 步星耀 张志文 西京医院神经外科(西安,710032);黄文晋 肖华胜 神经科学研究所

    关键词:脑肿瘤;SV40;大T抗原;抑癌蛋白;pRb

    癌症990416

    【摘要】目的:探讨SV40早期基因编码产物大T抗原(Tag)表达及与抑癌蛋白pRb的相互作用在人脑肿瘤发生发展中的意义。方法:采用免疫共沉淀及Western印迹法检测43例人脑肿瘤组织及5例正常人脑组织中Tag的表达,并对15例Tag阳性瘤组织检测Tag-pRb复合物的存在。结果:Tag在5例室管膜瘤及2例脉络丛乳头状瘤中全部表达,垂体腺瘤(5/6)、星形胶质细胞瘤(7/10)、脑膜瘤(4/6)、多形性胶质母细胞瘤(2/5)及髓母细胞瘤(1/5)均有Tag的表达;3例少枝胶质细胞瘤、1例松果体瘤及5例正常人脑组织无Tag表达。15例Tag阳性瘤组织中均发现Tag与pRb形成特异性复合物。结论:在人脑肿瘤组织中Tag广泛表达,Tag可与pRb形成特异性复合物,Tag-pRb特异性复合物的形成导致pRb失活,可能是人脑肿瘤发生发展的一个重要机理。

    中图分类号:R739.41 文献标识码:A 文章编号:1000-467X(1999)04-0409-03

    SV40 large tumor antigen forms a specific complex with pRb in human brain tumors

    ZHEN Hai-ning, ZHANG Xiang, BU Xing-yao, et al.

    Department of Neurosurgery,Xijing Hospital,The Fourth Military Medical University,Xi’an 710032,P.R.China

    【Abstract】 Objective:To research the role of SV40 early region gene coding product large tumor antigen (Tag) and the interaction between Tag and tumor suppressor pRb in human brain tumorigenesis Methods:Tag was investigated by immunoprecipitation and Western blot in 43 cases of human brain tumors and 5 cases of normal brain tissues.Tag-pRb specific complex was screened in 15 Tag positive cases.Results:Tag was found in all of 5 ependymomas and 2 choriod plexus papillomas, as well as in pituitary adenomas (5/6), astrocytomas (7/10),meningiomas (4/6),glioblastomas multiforme (2/5),medulloblastomas (1/5).None of the 5 normal brain tissues was positive for Tag,nor were 3 oligodendrogliomas and 1 pineocytoma. Tag-pRb complex was found in all of 15 Tag positive cases.Conclusions:SV40 Tag was expressed highly in human brain tumors,and can form a specific complex with pRb,the inactivation of pRb due to the formation of Tag-pRb complex may be an important mechanism in the etiopathogenesis of human brain tumors. ......

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