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VEGF及其受体flt对荷瘤鼠喉癌细胞增殖的影响
http://www.100md.com 《癌症》1999年第4期
喉肿瘤|血管内皮生长因子|增殖
     作者:邓志宏 黄维国 邱建华 林顺涨 金岩

    单位:第四军医大学西京医院耳鼻喉科(西安,710032)

    关键词:喉肿瘤;血管内皮生长因子;增殖

    癌症990417

    【摘要】目的:观察血管内皮生长因子(VEGF)对喉癌细胞增殖动力学的影响。方法:首先建立荷瘤(HEP-2喉癌细胞)裸鼠动物模型,将抗VEGF抗体及抗VEGF受体flt抗体分别注射荷瘤鼠体内,通过活体标记BrdU的方法,观察抗VEGF抗体及抗flt抗体对喉癌HEP-2细胞增殖的影响。结果:与对照组相比较,抗VEGF抗体治疗组HEP-2细胞BrdU标记阳性率显著降低(P<0.05);抗flt抗体治疗组BrdU标记阳性率亦显著小于对照组(P<0.05);而抗VEGF抗体治疗组与抗flt抗体治疗组BrdU标记率无显著性差异(P>0.05)。结论:中和VEGF抗体的作用及封闭VEGF结合位点均可抑制喉癌细胞的增殖,提示VEGF与喉癌细胞生长有着密切的关系。

    中图分类号:R739.6 文献标识码:A 文章编号:1000-467X(1999)04-0412-03

    Effect of anti-VEGF and its receptor antibody on the growth of laryngeal cancer Hep-2 cells in nude mice

    DENG Zhi-hong, HUANG Wei-guo,OIU Jian-hua, et al.

    Department of Otolaryngology and Department of Oral Pathology,Fourth Military Medical University,Xi’an 710032,P.R.China

    【Abstract】 Objective:To observe the effect of anti-VEGF and its receptor antibody on the growth of laryngeal carcer Hep-2 cell line.Methods:Establishing laryngeal cancer xenografts in nude mice, then treating the tumor-bearing mice with anti-VEGF and its receptor antibody, observing the effect of anti-VEGF and its receptor antibody on the growth of laryngeal carcer Hep-2 cells through labeling BrdU in vivo. Results:The number of BrdU labeled cells in the groups treated with MoAb was significantly lower than that of the control group, there were no diffecrences between the groups treated with anti-VEGF antibody and anti-VEGF receptor antibody.Conclusions:Both neutralizing VEGF antibody and blocking VEGF-biting site could inhibit the growth of laryngeal cancer cells, VEGF was highly associated with the growth of laryngeal cancer cells. ......

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