对正常及大细胞性改变的肝细胞、肝癌和癌旁改变肝细胞ICM-DNA定量研究
作者：陆良勇 陈成伟 傅爱芬 刘尚廉 顾康庭
单位：陆良勇 陈成伟(200233 解放军85医院/南京军区上海临床肝病研究中心分子病理室)；傅爱芬(上海第二医科大学病解教研室)；刘尚廉(上海医科大学肿瘤医院病理科)；顾康庭(上海交通大学图象处理与模式识别研究所)
关键词：癌旁改变肝细胞；大细胞性改变肝细胞；肝癌细胞；ICM-DNA定量
肝脏000103摘 要：目的探索正常肝细胞、大细胞性改变肝细胞、肝癌和癌旁改变肝细胞的DNA含量和形态特点。方法采用DNA组织化学染色和图像细胞分析术。结果10例癌旁改变(PC)组DNA指数(1.99±0.34)分别高于4例正常肝细胞(NH)组(0.98±0.12)和8例肝细胞的大细胞性改变(LCC)组(1.46±0.19)(P<0.01)，DNA倍体分布与NH、LCC和肝细胞癌(HCC)组存在差异(P<0.05)，核面积和核周长(74.0±52.3，63.9±33.9)低于26例HCC组(95.6±95.2，76.3±62.9)(P<0.01)。PC组中80%(8/10)为非整倍体DNA直方图表型，高于HCC组的53%(14/26)，而多倍体占20%(2/10)低于HCC组26.92%(7/26)(P<0.05)。PC肝细胞核异型，核质浓缩，胞质嗜酸性或嗜碱性与HCC有过渡，置换性生长方式又与HCC相同。结论呈片块状分布或腺瘤样排列的PC肝细胞，其异常DI、非整倍体分布和DNA直方图表型应作为癌前状态。
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DNA content study of normal hepatocytes,large cell change hepatocytes,hepatocellular carcinoma and hepatocytic peri-cancerous change in pericancerous parenchyma with image cytometry
LU Liangyong,CHEN Chengwei,FU Aifen,et al.
(Department of Molecular Pathology,85th Army Hospital,Shanghai Liver Diseases Research Center of Nanjing Area,Shanghai 200233)
Abstract：Objective To investigate the DNA content and morphologic characteristics of normal hepatocytes (NH),large cell change hepatocytes (LCC),hepatocellular carcinoma (HCC) and the hepatocytic pericancerous change (PC) in peri-cancerous parenchyma.Methods By use of histochemistry staining of DNA and image cytometry.Results In 10 cases of PC group,the DNA index (DI,1.99±0.34) were higher than that in 4 cases with NH group (0.98±0.12 ) and 8 cases with LCC group (1.46±0.19),P<0.01,the DNA ploidy pattern was also different as compared with that in NH and LCC group(P<0.05).The nucleus surface area and nuclear circumference (74.01±52.3, 63.89±33.9) were lower than those in 26 cases of HCC group (95.62±95.21,76.33±62.91),P<0.01.Among the PC group,80% (8/10) were of aneuploidy pat-tern,more than that seen in HCC group 53% (14/26),20% (2/10) were of polyploidy pattern,less than that in HCC group 26.92% (7/26),P<0.05.In the PC group,the hepatocytes were rich in nuclear chromatin and irregular nuclei shape,the cy-toplasmic cosinophilia or basophilic showed transitional change of HCC,replaceing growth pattern showed similar with HCC.Conclusion The abnormal DNA content,DI and the ploidy pattern of PC hepatocytes which is diffuse mass-like distribution and adenomatous arrangement showed precancrosis celles.
, 百拇医药
Keywords：Hepatocytic pericancerous change;Large cell change hepatocytes;Hepatocellular carcinoma;ICM-DNA quantitation
参考文献：
[1]Okuda K,Kamal G.Ishak (Eds):Neoplasms of the liver.Springer Verlag,1987.88-89.
[2]Su Q,Benner A,Hofmann WJ,et al.Human hepatic preneoplasia:phenotypes and proliferation kinetics of foci and nodules of altered hepatocytes and their relationship to liver cell dysplasia. Virch Arch 1997,431:391-406.
, http://www.100md.com
[3]Peter RL.Pathology of hepatocellular carcinoma In:Okuda K,Peters RL(eds) Hepatocellular carcinoma,New York,1976.107-168.
[4]Anthony PP.Vogel CL.Barker LF.Liver cell dysplasia a premalig-nant condition.J Clin Pathol,1973,26:217-223.
[5]Okita K,Kodama T,Harada T,et al.Early lesions and development of primary hepatocellular carcinoma in man-association with hepatitis B viral infection.Gastroenterologia JPN,1977,12:51-57.
, 百拇医药
[6]Le Bail,Belleannee G,Bernard PH,et al.Adenomatous hyperplasia in cirrhotic livers:Histological evaluation,cellular density,and prolife-rative activity of 35 macronodular lesions in the cirrhotic explants of 10 adult French patients.Hum Pathol,1995,26:897-906.
[7]Chieco P,Van Noorden C.Comparative image cytometric DNA ploidy of liver cell dysplasia and hepatocellular carcinoma.Mod Pathol,1996,9:84-86.
[8]Sampatanukul P,Mikuz G,Israsena S,et al.Cytomorphologic and DNA cytometric feature of hepatocellular carcinoma in fine needle as-pirates.Acta Cyto,1997,41:435-442.
, http://www.100md.com
[9]Saiki I,Une Y,Shimamura T,et al.DNA ploidy pattern in resected human hepatocellular carcinomas from the viewpoint of biological ma-lignant potential.JPN Cancer Chemother,1996,23:135-142.
[10]Kay EW,Mulcahy HE,Curran B,et al.An image analysis study of DNA content in early colorectal cancer.Eur J Cancer,1996,32A:612-616.
收稿日期：1999-10-20, http://www.100md.com
单位：陆良勇 陈成伟(200233 解放军85医院/南京军区上海临床肝病研究中心分子病理室)；傅爱芬(上海第二医科大学病解教研室)；刘尚廉(上海医科大学肿瘤医院病理科)；顾康庭(上海交通大学图象处理与模式识别研究所)
关键词：癌旁改变肝细胞；大细胞性改变肝细胞；肝癌细胞；ICM-DNA定量
肝脏000103摘 要：目的探索正常肝细胞、大细胞性改变肝细胞、肝癌和癌旁改变肝细胞的DNA含量和形态特点。方法采用DNA组织化学染色和图像细胞分析术。结果10例癌旁改变(PC)组DNA指数(1.99±0.34)分别高于4例正常肝细胞(NH)组(0.98±0.12)和8例肝细胞的大细胞性改变(LCC)组(1.46±0.19)(P<0.01)，DNA倍体分布与NH、LCC和肝细胞癌(HCC)组存在差异(P<0.05)，核面积和核周长(74.0±52.3，63.9±33.9)低于26例HCC组(95.6±95.2，76.3±62.9)(P<0.01)。PC组中80%(8/10)为非整倍体DNA直方图表型，高于HCC组的53%(14/26)，而多倍体占20%(2/10)低于HCC组26.92%(7/26)(P<0.05)。PC肝细胞核异型，核质浓缩，胞质嗜酸性或嗜碱性与HCC有过渡，置换性生长方式又与HCC相同。结论呈片块状分布或腺瘤样排列的PC肝细胞，其异常DI、非整倍体分布和DNA直方图表型应作为癌前状态。
, http://www.100md.com
DNA content study of normal hepatocytes,large cell change hepatocytes,hepatocellular carcinoma and hepatocytic peri-cancerous change in pericancerous parenchyma with image cytometry
LU Liangyong,CHEN Chengwei,FU Aifen,et al.
(Department of Molecular Pathology,85th Army Hospital,Shanghai Liver Diseases Research Center of Nanjing Area,Shanghai 200233)
Abstract：Objective To investigate the DNA content and morphologic characteristics of normal hepatocytes (NH),large cell change hepatocytes (LCC),hepatocellular carcinoma (HCC) and the hepatocytic pericancerous change (PC) in peri-cancerous parenchyma.Methods By use of histochemistry staining of DNA and image cytometry.Results In 10 cases of PC group,the DNA index (DI,1.99±0.34) were higher than that in 4 cases with NH group (0.98±0.12 ) and 8 cases with LCC group (1.46±0.19),P<0.01,the DNA ploidy pattern was also different as compared with that in NH and LCC group(P<0.05).The nucleus surface area and nuclear circumference (74.01±52.3, 63.89±33.9) were lower than those in 26 cases of HCC group (95.62±95.21,76.33±62.91),P<0.01.Among the PC group,80% (8/10) were of aneuploidy pat-tern,more than that seen in HCC group 53% (14/26),20% (2/10) were of polyploidy pattern,less than that in HCC group 26.92% (7/26),P<0.05.In the PC group,the hepatocytes were rich in nuclear chromatin and irregular nuclei shape,the cy-toplasmic cosinophilia or basophilic showed transitional change of HCC,replaceing growth pattern showed similar with HCC.Conclusion The abnormal DNA content,DI and the ploidy pattern of PC hepatocytes which is diffuse mass-like distribution and adenomatous arrangement showed precancrosis celles.
, 百拇医药
Keywords：Hepatocytic pericancerous change;Large cell change hepatocytes;Hepatocellular carcinoma;ICM-DNA quantitation
参考文献：
[1]Okuda K,Kamal G.Ishak (Eds):Neoplasms of the liver.Springer Verlag,1987.88-89.
[2]Su Q,Benner A,Hofmann WJ,et al.Human hepatic preneoplasia:phenotypes and proliferation kinetics of foci and nodules of altered hepatocytes and their relationship to liver cell dysplasia. Virch Arch 1997,431:391-406.
, http://www.100md.com
[3]Peter RL.Pathology of hepatocellular carcinoma In:Okuda K,Peters RL(eds) Hepatocellular carcinoma,New York,1976.107-168.
[4]Anthony PP.Vogel CL.Barker LF.Liver cell dysplasia a premalig-nant condition.J Clin Pathol,1973,26:217-223.
[5]Okita K,Kodama T,Harada T,et al.Early lesions and development of primary hepatocellular carcinoma in man-association with hepatitis B viral infection.Gastroenterologia JPN,1977,12:51-57.
, 百拇医药
[6]Le Bail,Belleannee G,Bernard PH,et al.Adenomatous hyperplasia in cirrhotic livers:Histological evaluation,cellular density,and prolife-rative activity of 35 macronodular lesions in the cirrhotic explants of 10 adult French patients.Hum Pathol,1995,26:897-906.
[7]Chieco P,Van Noorden C.Comparative image cytometric DNA ploidy of liver cell dysplasia and hepatocellular carcinoma.Mod Pathol,1996,9:84-86.
[8]Sampatanukul P,Mikuz G,Israsena S,et al.Cytomorphologic and DNA cytometric feature of hepatocellular carcinoma in fine needle as-pirates.Acta Cyto,1997,41:435-442.
, http://www.100md.com
[9]Saiki I,Une Y,Shimamura T,et al.DNA ploidy pattern in resected human hepatocellular carcinomas from the viewpoint of biological ma-lignant potential.JPN Cancer Chemother,1996,23:135-142.
[10]Kay EW,Mulcahy HE,Curran B,et al.An image analysis study of DNA content in early colorectal cancer.Eur J Cancer,1996,32A:612-616.
收稿日期：1999-10-20, http://www.100md.com