大鼠皮肤损伤后纤维连接蛋白剪接异型体的表达
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法医学杂志 2000年第2期第16卷 论著
作者：刘宁国 赵子琴 陈忆九 顾云菊
单位：陈忆九(司法部司法鉴定科学技术研究所，上海200063)；刘宁国 赵子琴 顾云菊(上海医科大学，上海：200032)
关键词：纤维连接蛋白；损伤时间推断；地高辛；原位杂交
法医学杂志000203摘 要：运用地高辛标记纤维连接蛋白(FN)特殊位点的探针，通过原位杂交方法检测不同损伤时间大鼠皮肤各型FN剪接异型体的表达情况，结果发现：伤后大鼠皮肤各型FN的表达随时间呈增高趋势；伤后30-60min表达出正常皮肤中没有的EⅢA、EⅢB片段。PN的这一特性为法医学诊断生前、死后伤和伤后存活时间推断提供了新指标。
分类号：DF795.1 文献标识码：A
文章编号：1004-5619(2000)02-0070-02
, http://www.100md.com
The expression of splicing fibranectin variants of cutaneous injuries in rats
Liu Ningguo
(Shanghai Medical University,Shanghai 200032, P, R. China)
Abstract：In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, E Ⅲ A and E Ⅲ B Variants) in rats, The results showed that all forms of FN variants increased notably with the wounding time going on. E ⅢA and E Ⅲ B Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin.
, 百拇医药
Keywords：fibronectin; estimation of wounding interval; digoxingenin; in situ hybridization
基金项目：国家自然科学基金资助项目(No.39770816)
参考文献：
[1]赵子琴、张其英，陈忆九.对人和鼠腹部皮肤切创缘壁伤后不同时间纤维连接蛋白的变化的研究[J]. 法医学杂志，1993，9(3)：105-108.
[2]赵子琴，陈忆九，张其英，等.人体皮肤各类创伤区纤维连接蛋白免疫纽化的研究[J].法医学杂志,1996，15(2)：67-68.
[3]Kornblihtt AR, Vibe- Pedersen R, Paralle FE. et al. Human fibronectin: molecular cloning evidence for two mRNA species suffering by an internal segment coding for a structural domain[J]. EMBO J, 1984, 3:221-226.
, http://www.100md.com
[4]Kornblihtt AR, Vibe-Pedersen R, ParaUeb FE, et al. Human fibronectin: cell- specific alternative splicing generates polypeptide chains differing in the number of internal repeats[J] . Nucleic Acids Res, 1984, 12: 5853- 5856.
[5]Schwarzabauer JE , Patel RS , Fonda D. Multiple sites of alternative splicing of the rat fibronectin gene transcript[J] .EMBO J, 1987, 6: 2573-2580.
[6]Pagani F, Zagato L, Vergani C, et al. Tissuespecific pattern of fibronectin messenger RNA precursor during developmem aging in rat[J] .J Cell Biol, 1991, 113: 1223- 1229.
[7]Ffrench-Constant C, Van Der Water L, Dvorak HF, et al. Reappearance of an embryonic pattern of fibronectin splicing during wound healing in the adult rat[J]. J Cell Biol, 1989, 109:903-914.
收稿日期：1999-08-17
修稿日期：1999-10-10, 百拇医药
单位：陈忆九(司法部司法鉴定科学技术研究所，上海200063)；刘宁国 赵子琴 顾云菊(上海医科大学，上海：200032)
关键词：纤维连接蛋白；损伤时间推断；地高辛；原位杂交
法医学杂志000203摘 要：运用地高辛标记纤维连接蛋白(FN)特殊位点的探针，通过原位杂交方法检测不同损伤时间大鼠皮肤各型FN剪接异型体的表达情况，结果发现：伤后大鼠皮肤各型FN的表达随时间呈增高趋势；伤后30-60min表达出正常皮肤中没有的EⅢA、EⅢB片段。PN的这一特性为法医学诊断生前、死后伤和伤后存活时间推断提供了新指标。
分类号：DF795.1 文献标识码：A
文章编号：1004-5619(2000)02-0070-02
, http://www.100md.com
The expression of splicing fibranectin variants of cutaneous injuries in rats
Liu Ningguo
(Shanghai Medical University,Shanghai 200032, P, R. China)
Abstract：In situ hybridization with Digoxingenin labeled probes were used to build the relationship between incised cutaneous wounding intervals and the expression of splicing fibronectin variants (total, E Ⅲ A and E Ⅲ B Variants) in rats, The results showed that all forms of FN variants increased notably with the wounding time going on. E ⅢA and E Ⅲ B Variants were expressed abundantly 30 and 60 minutes after injury, while few in normal skin.
, 百拇医药
Keywords：fibronectin; estimation of wounding interval; digoxingenin; in situ hybridization
基金项目：国家自然科学基金资助项目(No.39770816)
参考文献：
[1]赵子琴、张其英，陈忆九.对人和鼠腹部皮肤切创缘壁伤后不同时间纤维连接蛋白的变化的研究[J]. 法医学杂志，1993，9(3)：105-108.
[2]赵子琴，陈忆九，张其英，等.人体皮肤各类创伤区纤维连接蛋白免疫纽化的研究[J].法医学杂志,1996，15(2)：67-68.
[3]Kornblihtt AR, Vibe- Pedersen R, Paralle FE. et al. Human fibronectin: molecular cloning evidence for two mRNA species suffering by an internal segment coding for a structural domain[J]. EMBO J, 1984, 3:221-226.
, http://www.100md.com
[4]Kornblihtt AR, Vibe-Pedersen R, ParaUeb FE, et al. Human fibronectin: cell- specific alternative splicing generates polypeptide chains differing in the number of internal repeats[J] . Nucleic Acids Res, 1984, 12: 5853- 5856.
[5]Schwarzabauer JE , Patel RS , Fonda D. Multiple sites of alternative splicing of the rat fibronectin gene transcript[J] .EMBO J, 1987, 6: 2573-2580.
[6]Pagani F, Zagato L, Vergani C, et al. Tissuespecific pattern of fibronectin messenger RNA precursor during developmem aging in rat[J] .J Cell Biol, 1991, 113: 1223- 1229.
[7]Ffrench-Constant C, Van Der Water L, Dvorak HF, et al. Reappearance of an embryonic pattern of fibronectin splicing during wound healing in the adult rat[J]. J Cell Biol, 1989, 109:903-914.
收稿日期：1999-08-17
修稿日期：1999-10-10, 百拇医药