大鼠脑线粒体DNA提取方法
作者：项平 高国全 姚志彬
单位：项平(蚌埠医学院解剖教研室 蚌埠233000)；高国全 姚志彬(中山医科大学脑研究室)
关键词：线粒体DNA；大鼠；聚合酶链式反应
解剖科学进展000227摘 要：本研究用一种简便方法提取大鼠脑线粒体DNA(mtDNA)，所得的线粒体DNA纯度为1.8±0.01，得率为每克脑组织0.8～1.0μg线粒体DNA，以此为模板进行PCR反应，扩增线粒体DNA编码基因，产量丰富、特异性高。该方法操作简便、成本低，适用于一般实验室。 Isolation of the Mitochondrial DNA from Rat Brain
Xiang Ping ,Gao Guoquan ,Yao Zhibin
(Department of Anatomy, Bengbu Medical College, Bengbu, 233003)
Abstract：A simple technique for isolation of mitochondrial DNA from rat brain is described. The purity (260/280) of mtDNA extracted by the method is 1.8±0. 01, the mtDNA yield per gram brain is 0. 8-10/μg. The product of the PCR is specific. The method can be used in general laboratory because it costs very low and is easy to operate.
Keywords：mtDNA; rat; PCR
参考文献：
[1]Bibb JM, Van Etten RA, Wright CT, et al. Sequence and gene organization of mouse mitochondrial DNA. Cell, 1981,26:167
[2]Schon EA, Rizzuto R, Moreas CT, et al. A direct repeat is ahot-spot for large-scale deletion of human mltochondrial DNA.Science, 1989,244: 346
[3]吴鹤龄，林锦湖.遗传实验方法.北京，高等教育出版社，1983，108-114
[4]Gadaleta P, Pepe G, Candia D, et al. The complete nuclueotide sequence of the rattus norvegicus mitochondrial geneome : cryptic signal revealed by comparative analysis between vertebrated. J Mol Evol, 1989,28:497
[5]江谦.现代医学实验方法.北京，人民卫生出版社，1997，335-350, 百拇医药
单位：项平(蚌埠医学院解剖教研室 蚌埠233000)；高国全 姚志彬(中山医科大学脑研究室)
关键词：线粒体DNA；大鼠；聚合酶链式反应
解剖科学进展000227摘 要：本研究用一种简便方法提取大鼠脑线粒体DNA(mtDNA)，所得的线粒体DNA纯度为1.8±0.01，得率为每克脑组织0.8～1.0μg线粒体DNA，以此为模板进行PCR反应，扩增线粒体DNA编码基因，产量丰富、特异性高。该方法操作简便、成本低，适用于一般实验室。 Isolation of the Mitochondrial DNA from Rat Brain
Xiang Ping ,Gao Guoquan ,Yao Zhibin
(Department of Anatomy, Bengbu Medical College, Bengbu, 233003)
Abstract：A simple technique for isolation of mitochondrial DNA from rat brain is described. The purity (260/280) of mtDNA extracted by the method is 1.8±0. 01, the mtDNA yield per gram brain is 0. 8-10/μg. The product of the PCR is specific. The method can be used in general laboratory because it costs very low and is easy to operate.
Keywords：mtDNA; rat; PCR
参考文献：
[1]Bibb JM, Van Etten RA, Wright CT, et al. Sequence and gene organization of mouse mitochondrial DNA. Cell, 1981,26:167
[2]Schon EA, Rizzuto R, Moreas CT, et al. A direct repeat is ahot-spot for large-scale deletion of human mltochondrial DNA.Science, 1989,244: 346
[3]吴鹤龄，林锦湖.遗传实验方法.北京，高等教育出版社，1983，108-114
[4]Gadaleta P, Pepe G, Candia D, et al. The complete nuclueotide sequence of the rattus norvegicus mitochondrial geneome : cryptic signal revealed by comparative analysis between vertebrated. J Mol Evol, 1989,28:497
[5]江谦.现代医学实验方法.北京，人民卫生出版社，1997，335-350, 百拇医药