西洋参DNA导入大豆实现遗传转化的研究Ⅱ.导入后代异黄酮类成分的含量测定
作者：刘塔斯 麻浩 田森林 川村智子 田中俊弘
单位：刘塔斯(湖南中医学院中药系，长沙 410007)；麻浩 田森林(湖南农业大学植物科技学院)；川村智子(日本名城大学生药学教研室)；田中俊弘(日本岐阜药科大学药草园研究室)
关键词：西洋参DNA导入大豆后代；大豆苷；大豆黄素；染料木苷；染料木素；HPLC法
中草药000211 摘 要 用高效液相色谱法测定了西洋参DNA导入大豆后代的异黄酮类成分、大豆苷(daidzin)、大豆黄素(daidzein)、染料木苷(genistin)、染料木素(genistein)的含量，采用Unisil Q C18柱，4.6 mm×250 mm，流动相乙腈-0.003 mol/L磷酸氢二钠(17∶83)，流速1.0 mL/min，检测波长254 nm，结果表明，4种成分分离效果好，成分含量发生了广泛变异，从中可筛选出有效成分含量较高的新材料。
, 百拇医药
Genetic Transformation of Soybeans by Soaking Its Seeds with the Total DNA of American Ginseng (Panax quinquefolium) Ⅱ. Determination of Isoflavone Content by HPLC
Liu Tasi
(Department of Chinese Materia Medica, Hunan College of Traditional Chinese Medicine Changsha 410007)
Ma Hao and Tian Senlin
(College of Plant Science and Technology, Hunan Agriculture University)
, 百拇医药
Tomoko Kawamora
(Department of Pharmacognosy, Meijo University, Meijo, Japan)
Toshihiro Tanaka
(Gifu Pharmaceutical University, Japan)
Abstract A method for the determination of isoflavone content in the transformed progenies of soybean by HPLC was reported. Using unisil Q C18 column (4.6 mm×250 mm, 5 μm), with a mobile phase of CH3CN-0.003 mol/L Na2HPO4 (17∶83), a detecting wavelength at 254 nm, and flow rate of 1.0 mL/min,it was found that the contents of daidzein, genistein, daidzin and genistin in the transformed progenies of soybean changed greatly and could be separated effectively, which makes it possible to cultivate and develop the best progeny for further development.
, http://www.100md.com
Key words the transformed progenies of soybean with the total DNA of American Ginseng daidzin daidzein genistin genistein HPLC▲
我们已知道了将西洋参DNA导入大豆后代的形态性状变化[1]
单位：刘塔斯(湖南中医学院中药系，长沙 410007)；麻浩 田森林(湖南农业大学植物科技学院)；川村智子(日本名城大学生药学教研室)；田中俊弘(日本岐阜药科大学药草园研究室)
关键词：西洋参DNA导入大豆后代；大豆苷；大豆黄素；染料木苷；染料木素；HPLC法
中草药000211 摘 要 用高效液相色谱法测定了西洋参DNA导入大豆后代的异黄酮类成分、大豆苷(daidzin)、大豆黄素(daidzein)、染料木苷(genistin)、染料木素(genistein)的含量，采用Unisil Q C18柱，4.6 mm×250 mm，流动相乙腈-0.003 mol/L磷酸氢二钠(17∶83)，流速1.0 mL/min，检测波长254 nm，结果表明，4种成分分离效果好，成分含量发生了广泛变异，从中可筛选出有效成分含量较高的新材料。
, 百拇医药
Genetic Transformation of Soybeans by Soaking Its Seeds with the Total DNA of American Ginseng (Panax quinquefolium) Ⅱ. Determination of Isoflavone Content by HPLC
Liu Tasi
(Department of Chinese Materia Medica, Hunan College of Traditional Chinese Medicine Changsha 410007)
Ma Hao and Tian Senlin
(College of Plant Science and Technology, Hunan Agriculture University)
, 百拇医药
Tomoko Kawamora
(Department of Pharmacognosy, Meijo University, Meijo, Japan)
Toshihiro Tanaka
(Gifu Pharmaceutical University, Japan)
Abstract A method for the determination of isoflavone content in the transformed progenies of soybean by HPLC was reported. Using unisil Q C18 column (4.6 mm×250 mm, 5 μm), with a mobile phase of CH3CN-0.003 mol/L Na2HPO4 (17∶83), a detecting wavelength at 254 nm, and flow rate of 1.0 mL/min,it was found that the contents of daidzein, genistein, daidzin and genistin in the transformed progenies of soybean changed greatly and could be separated effectively, which makes it possible to cultivate and develop the best progeny for further development.
, http://www.100md.com
Key words the transformed progenies of soybean with the total DNA of American Ginseng daidzin daidzein genistin genistein HPLC▲
我们已知道了将西洋参DNA导入大豆后代的形态性状变化[1]