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牛膝提取物对成骨样细胞增殖的作用
http://www.100md.com 《沈阳药科大学学报》2000年第3期
牛膝|促细胞增殖作用|成骨样细胞UMR106|MTT法
     作者:高晓燕 王大为 李发美 姜志明

    单位:王大为 高晓燕(沈阳药科大学基础部,沈阳 110015)

    关键词:牛膝;促细胞增殖作用;成骨样细胞UMR106;MTT法

    沈阳药科大学学报000318

    摘 要 考察了牛膝(Achyranthes bidentata Bl.)的不同提取部位对成骨样细胞UMR106的作用.通过牛膝的醇提液及醇提液的不同提取部位和UMR106成骨样细胞共同体外培养,用MTT方法检测了各部位对细胞增殖的作用;同时考察了MTT法的实验条件.结果表明:牛膝的醇提液及其石油醚和乙酸乙酯萃取的混和物对细胞具有较强的促进增殖作用,而正丁醇层和水层对细胞增殖无影响;在MTT法中,铺板细胞浓度、检测波长和加入MTT后的孵育时间均影响吸光度值的测定.牛膝中低极性部位可能含有直接作用于成骨细胞的活性成分.

    分类号 R97.2

    The Effect of the Extracts of Achyranthes bidentata

    Bl. onProliferation of Osteoblast-like UMR 106 Cells

    Gao Xiaoyan,Wang Dawei,Li Famei,Jiang Zhiming

    Department of Basic Courses,Shenyang Pharmaceutical University,Shenyang 110015

    Abstract The effects of the extracts of Achyranthes bidentata Bl.(AB)on osteoblast-like(OB-like) UMR 106cells were studied.The extracts were incubated with cells in vitro.The proliferation of OB-like cells wasdetermined by using the MTT assay,and the assay conditions were investigated.It was found that theconcentration of cells,determination of the wavelength as well as cultured time with the MTT solution affected themeasurement of absorbance.The ethanol(75%)extract and the mixture extracted with petroleum ether and ethylacetate showed the stimulation activity to OB-like cells.The results indicated that the lower-polar substance ofAB may contain constituents active in stimulating osteoblast. ......

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