肾综合征出血热病毒在体外培养的血管内皮细胞中增殖的研究
作者：姚磊 盛鹏 朱德钟
单位：姚磊(第三军医大学新桥医院检验科(重庆400037))；盛鹏(第三军医大学微生物教研室)；朱德钟(第三军医大学微生物教研室)
关键词：肾综合征出血热；血管内皮细胞；微量免疫酶斑法
重庆医学000404摘 要：目的研究肾综合征出血热(HFRS)病毒在血管内皮细胞(HEC)内的增殖。方法采用微量免疫酶斑法检测HFRS病毒(A9株、PUV株)感染后HEC的病毒繁殖动态，并与病毒在Vero-E6细胞繁殖动态相互对比。结果HFRS病毒感染HEC后第一天即可检验到病毒释放，A9株第3天达增殖高峰，RUV株第7天达增殖高峰。结论HFRS病毒可在原始靶细胞HEC内增殖，但不同的病毒株繁殖速度不同，这种差异可能与其致病力有关。
Multiplication of HFRS virus in HECs in vitro
, http://www.100md.com
YaoLei
(Department of Clinical Laboratory Medicine, Xinqiao Hospital, Third military Medical University, Chongqing 400037)
Sheng Peng ,Zhu Dezhong
(Department of Microbiology Third Military Medical University)
Abstract：Aim:To study the multiplication of hemorrhagic fever with renal syndrome(HFRS) virus in human endothelial cells(HEC) and veto-E6 cells. Methods:HFRS pathogenic strain (A9)and non-pathogenic strain(PHV)infected HECs, at different time after infection (1d, 3d,5d 7d 9d), the titers of virus were measured by micro immunoenzymatic plaque method.Results:HEC infected with,A9 strain produced infectious virus during the first 3 days to a peak and declined later, this was contrast with the PHV growth pattern observed in HEC, which showed increasing yields of virus up to 5 day after infection. Coneluaion:HEC as HFRS virus original target cell can produce infective virus. But there were different grow patterns in different strains and host cells, This diversity may be associated with HERS virus infective activity.
, 百拇医药
Keywords：hemorrhagic fever with renal syndrome virus jhuman endothelial cells microimmunoenzymic plague method
参考文献：
[1]Kuratat T,Tsai TF，Bauer SB，et al.Immunofluorescence studies fo disseminated Hantaan Virus infection of sucking mice.Infect Immun，1983，41(1)：391
[2]姚磊，盛鹏，朱德钟.培养人肺静脉血管内皮细胞的研究.实用医技杂志，1999，6(12)：915
[3]刘启富，朱德钟，王宁，等.微斑免疫酶技术在检测肾综合征出血热病毒及其特异抗体中的应用.免疫学杂志，1990，6(3)：198
, 百拇医药
[4]杭长寿.流行性出血热病毒分子生物学研究.中华媒介生物学及控制杂志，1994，(特1)：6
[5]Yon YC，Yeshimatsu K，Yoshida，et al.Comparison of virulence between seoul virus strain SR-11 and hantaan virus strain 76-118 of havntavirus in newborn mice.Microbiol Immunol，1993，37(7)：557
[6]Pensiero MN，Sharefkin JB，Dieffenbach CW，et al.Hantaan virus infection of human endothelial cells J Virol，1992，66(10)：5929, 百拇医药
单位：姚磊(第三军医大学新桥医院检验科(重庆400037))；盛鹏(第三军医大学微生物教研室)；朱德钟(第三军医大学微生物教研室)
关键词：肾综合征出血热；血管内皮细胞；微量免疫酶斑法
重庆医学000404摘 要：目的研究肾综合征出血热(HFRS)病毒在血管内皮细胞(HEC)内的增殖。方法采用微量免疫酶斑法检测HFRS病毒(A9株、PUV株)感染后HEC的病毒繁殖动态，并与病毒在Vero-E6细胞繁殖动态相互对比。结果HFRS病毒感染HEC后第一天即可检验到病毒释放，A9株第3天达增殖高峰，RUV株第7天达增殖高峰。结论HFRS病毒可在原始靶细胞HEC内增殖，但不同的病毒株繁殖速度不同，这种差异可能与其致病力有关。
Multiplication of HFRS virus in HECs in vitro
, http://www.100md.com
YaoLei
(Department of Clinical Laboratory Medicine, Xinqiao Hospital, Third military Medical University, Chongqing 400037)
Sheng Peng ,Zhu Dezhong
(Department of Microbiology Third Military Medical University)
Abstract：Aim:To study the multiplication of hemorrhagic fever with renal syndrome(HFRS) virus in human endothelial cells(HEC) and veto-E6 cells. Methods:HFRS pathogenic strain (A9)and non-pathogenic strain(PHV)infected HECs, at different time after infection (1d, 3d,5d 7d 9d), the titers of virus were measured by micro immunoenzymatic plaque method.Results:HEC infected with,A9 strain produced infectious virus during the first 3 days to a peak and declined later, this was contrast with the PHV growth pattern observed in HEC, which showed increasing yields of virus up to 5 day after infection. Coneluaion:HEC as HFRS virus original target cell can produce infective virus. But there were different grow patterns in different strains and host cells, This diversity may be associated with HERS virus infective activity.
, 百拇医药
Keywords：hemorrhagic fever with renal syndrome virus jhuman endothelial cells microimmunoenzymic plague method
参考文献：
[1]Kuratat T,Tsai TF，Bauer SB，et al.Immunofluorescence studies fo disseminated Hantaan Virus infection of sucking mice.Infect Immun，1983，41(1)：391
[2]姚磊，盛鹏，朱德钟.培养人肺静脉血管内皮细胞的研究.实用医技杂志，1999，6(12)：915
[3]刘启富，朱德钟，王宁，等.微斑免疫酶技术在检测肾综合征出血热病毒及其特异抗体中的应用.免疫学杂志，1990，6(3)：198
, 百拇医药
[4]杭长寿.流行性出血热病毒分子生物学研究.中华媒介生物学及控制杂志，1994，(特1)：6
[5]Yon YC，Yeshimatsu K，Yoshida，et al.Comparison of virulence between seoul virus strain SR-11 and hantaan virus strain 76-118 of havntavirus in newborn mice.Microbiol Immunol，1993，37(7)：557
[6]Pensiero MN，Sharefkin JB，Dieffenbach CW，et al.Hantaan virus infection of human endothelial cells J Virol，1992，66(10)：5929, 百拇医药