TIMP-2拮抗胃癌SGC-7901 细胞侵袭能力实验研究
作者：李楠 徐采朴 刘为纹 杨金亮
单位：李楠(解放军309医院消化内科(北京100091))；徐采朴 刘为纹 杨金亮(第三军医大学西南医院消化内科(重庆400038))
关键词：胃肿瘤；侵袭；基质金属蛋白酶抑制-2
重庆医学000405摘 要：目的探讨基质金属蛋白酶抑制剂-2(TIMP-2)对SGC-7901胃癌细胞体外侵袭能力的影响。方法将人全长TIMP-2基因经脂质包裹转染PA317细胞系，G418筛选，感染人SCG-7901胃癌细胞系，经体外细胞增殖、细胞电泳率、软琼脂形成和侵袭小室的实验综合分析其生物学行为改变。结果经TIMP-2基因转染的SGC-7901细胞和电泳率、软琼脂形成和侵袭能力均明显低于对照组，而与对照组相比细胞增殖曲线没有明显的变化。结论TIMP-2基因转染可以使肿瘤细胞的表面电荷发生变化，使细胞的电泳能力、细胞的移动性和侵袭穿透能力减弱，而与细胞和生长周期没有明显影响。提示TIMP-2基因可能是通过影响细胞的外环境来抑制瘤细胞的恶性生物学表型。
, 百拇医药
Studies on the Anti-invasive Character of TIMP-2 Gene Transfected Into Gastric Carcinoma Cell SCG-7901
Li Nan
(Department of Gastroenterology, 309 Hospitol, Chinese PLA, Beijing 100091)
Xu Cai pu ,Liu Wei wen ,Yang jing liang
(Department of Gastroenterology, Southwest Hospitol, Third Military Medical University, Chongqing 430038, China)
, 百拇医药
Abstract：Aim:To invastigate the in vitro change of invason of gastric carcinoma cell SGC-7901 transfected with matrix metalloproteinase2 (TIMP-2)gene. Methods: A full human TIMP-2 cDNA gene was constructed into virus vehicle PT-MT, and was transferred into human gastric carcinoma cells SGC-7901 and positive clone was established by Boyden chambex in vitro. Results:The expression of TIMP-2 gene in SGC-7901-T2 cells was higher than that in the SCG-7901 cells in vitro and the ability of invasion in Boyden chamber was weak in vitro.The cell proliferation curves of TIMP-2 transfected and TIMP-2 untransfected groups had no obvious difference. Conclusion:The TIMP-2 gene may be an important negative factor in gastric carcinoma cell invasion and metastasis.
, 百拇医药
Keywords：Gastric neoplasm;Invasion;metalloproteinase-2
参考文献：
[1]李楠，王鑫，冯东晓，等.TIMP-2 基因逆转录病毒载体的构建.军事医学科学院院刊，1996，1:180
[2]Lenegal E,Gum R, Juarez j, et al. Induction of Mr, 92,000 type IV collagenase expression in a squamous carcinoma cell lines by fibroblasts.Cancer Res,1995, 67(10):963
[3]Carlod PS, Norman HA, Parmender P, et al. Prostate cancer progression, Metastasis, and gene expression in transfenic mine. Cancer Res, 1997, 57(5):900
, 百拇医药
[4]Hcc J, Sood B, Hoe Y, et al. Anti-invasive activity of matrix metalloproteinase-9(MMP-9) in TH-1080 human fibrosarcoma calls. Cancer Res, 1995, 56(10):2218
[5]Takahia T, Hiroshy S, Motoharm S, et al. Molecular biology of matrixmetallopoteinase (MMPs) and tissue inbibitor of metalloproteinases(TIMPs). and the regulations of these genes in tumortissues.Jap Jap J Clin Med, 1995, 53:179
[6]Albili Y, Klemen HK, Sommen GH, et al. A rapid in vitro assay for qualitive the invasion protential of tumor cell.Cancer Res, 1987,47:3229, 百拇医药
单位：李楠(解放军309医院消化内科(北京100091))；徐采朴 刘为纹 杨金亮(第三军医大学西南医院消化内科(重庆400038))
关键词：胃肿瘤；侵袭；基质金属蛋白酶抑制-2
重庆医学000405摘 要：目的探讨基质金属蛋白酶抑制剂-2(TIMP-2)对SGC-7901胃癌细胞体外侵袭能力的影响。方法将人全长TIMP-2基因经脂质包裹转染PA317细胞系，G418筛选，感染人SCG-7901胃癌细胞系，经体外细胞增殖、细胞电泳率、软琼脂形成和侵袭小室的实验综合分析其生物学行为改变。结果经TIMP-2基因转染的SGC-7901细胞和电泳率、软琼脂形成和侵袭能力均明显低于对照组，而与对照组相比细胞增殖曲线没有明显的变化。结论TIMP-2基因转染可以使肿瘤细胞的表面电荷发生变化，使细胞的电泳能力、细胞的移动性和侵袭穿透能力减弱，而与细胞和生长周期没有明显影响。提示TIMP-2基因可能是通过影响细胞的外环境来抑制瘤细胞的恶性生物学表型。
, 百拇医药
Studies on the Anti-invasive Character of TIMP-2 Gene Transfected Into Gastric Carcinoma Cell SCG-7901
Li Nan
(Department of Gastroenterology, 309 Hospitol, Chinese PLA, Beijing 100091)
Xu Cai pu ,Liu Wei wen ,Yang jing liang
(Department of Gastroenterology, Southwest Hospitol, Third Military Medical University, Chongqing 430038, China)
, 百拇医药
Abstract：Aim:To invastigate the in vitro change of invason of gastric carcinoma cell SGC-7901 transfected with matrix metalloproteinase2 (TIMP-2)gene. Methods: A full human TIMP-2 cDNA gene was constructed into virus vehicle PT-MT, and was transferred into human gastric carcinoma cells SGC-7901 and positive clone was established by Boyden chambex in vitro. Results:The expression of TIMP-2 gene in SGC-7901-T2 cells was higher than that in the SCG-7901 cells in vitro and the ability of invasion in Boyden chamber was weak in vitro.The cell proliferation curves of TIMP-2 transfected and TIMP-2 untransfected groups had no obvious difference. Conclusion:The TIMP-2 gene may be an important negative factor in gastric carcinoma cell invasion and metastasis.
, 百拇医药
Keywords：Gastric neoplasm;Invasion;metalloproteinase-2
参考文献：
[1]李楠，王鑫，冯东晓，等.TIMP-2 基因逆转录病毒载体的构建.军事医学科学院院刊，1996，1:180
[2]Lenegal E,Gum R, Juarez j, et al. Induction of Mr, 92,000 type IV collagenase expression in a squamous carcinoma cell lines by fibroblasts.Cancer Res,1995, 67(10):963
[3]Carlod PS, Norman HA, Parmender P, et al. Prostate cancer progression, Metastasis, and gene expression in transfenic mine. Cancer Res, 1997, 57(5):900
, 百拇医药
[4]Hcc J, Sood B, Hoe Y, et al. Anti-invasive activity of matrix metalloproteinase-9(MMP-9) in TH-1080 human fibrosarcoma calls. Cancer Res, 1995, 56(10):2218
[5]Takahia T, Hiroshy S, Motoharm S, et al. Molecular biology of matrixmetallopoteinase (MMPs) and tissue inbibitor of metalloproteinases(TIMPs). and the regulations of these genes in tumortissues.Jap Jap J Clin Med, 1995, 53:179
[6]Albili Y, Klemen HK, Sommen GH, et al. A rapid in vitro assay for qualitive the invasion protential of tumor cell.Cancer Res, 1987,47:3229, 百拇医药