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硝苯地平和罗钙全对尿毒症患者甲状旁腺素受体基因表达的影响
http://www.100md.com 《中华肾脏病杂志》 2000年第5期
     作者:陈靖 顾勇 张健 朱蔚钰 林善锬

    单位:200040 上海医科大学华山医院肾内科

    关键词:尿毒症;受体,甲状旁腺素;钙通道阻滞剂;维生素D3

    中华肾脏病杂志000503摘 要:目的观察钙通道阻滞剂硝苯地平和活性维生素D制剂罗钙全对尿毒症患者外周血单个核细胞(PBMC)上甲状旁腺素(PTH)受体基因表达的影响。方法30例非糖尿病常规血液透析患者随机分成硝苯地平组、罗钙全组和安慰剂组,治疗8周。应用逆转录,聚合酶链反应(RT-PCR)方法检测用药前后PBMC上PTH受体Mrna表达。结果硝苯地平治疗能使尿毒症患者PBMc上PTH受体Mrna的低表达显著上调(0.1436±0.0500比0.3584±0.0400,P<0.001),但仍未达到正常组水平(0.6837±0.0300,P<0.001)。罗钙全治疗可明显纠正低钙、高磷和高PTH血症,对PTH受体Mrna表达也有一定上调作用(0.1472±0.0100比0.2192±0.0200,P<0.001)。结论硝苯地平和罗钙全均能部分纠正尿毒症患者PTH受体基因表达的异常。
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    Effects of nifedipine and calcitriol on the PTH/PTHrP receptor mRNA expression in uremic patients

    CHEN Jing ,GU Yong ,ZHANG Jian,et al.

    (Division of Nephrology,Huashan Hospital,Shanghai Medical University,Shanghai 200040,China)

    Abstract:Objective To investigate the effects of calcium channel blocker (nifedipine)and 1,25 (OH)2VitD3 (calcitriol)on the mRNA expression of PTH receptor in PBMC of uremic patients.Methods Thirty non-diabetic uremic patients undergoing maintenance hemodialysis were divided into the following groups:(1)nifedipine group;(2)calcitriol group;(3)placebo group.The level of PTH receptor mRNA was measured by semi-quantitative RT-PCR method at the end of 8th week.Results The clown-regulation of PTH receptor in PBMC was observed in all patients before treatment,and there was no difference among three groups.After 8-week treatment,the levels of PTH receptor mRNA expression(PTH receptor I.O.D/beta-actin I.O.D) were significantly increased in nifedipine group(from 0.1436±0.0500 to 0. 3584±0. 0400,P<0.001 )and in calcitriol group(from 0.1472±0.0100 to 0.2192±0.0200,P<0.001),but still lower than that in normal group(0.6837±0.0300,P<0.001).Placebo therapy produced no change.Conclusion Both calcium channel blocker and 1,25(OH)2VitD3 could up-regulate PTH receptor mRNA expression in PBMC of uremic patients.
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    Keywords:Uremia;Receptor,parathyroid hormone;Calcium channel blocker;1,25 (OH) 2VitD3基金项目:卫生部基金资助项目(96-2-127)

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    收稿日期:2000-04-17, 百拇医药