黄芪当归合剂防治肾病综合征鼠进行性肾小管间质损伤
作者：余凌 张俊峰 李惊子 周安宇 邹万忠 王海燕
单位：100034 北京大学第一医院肾内科 肾脏病研究所
关键词：黄芪；当归；α-肌动蛋白；肾小管间质
中华肾脏病杂志000502摘 要：目的探讨黄芪当归合剂对肾病综合征鼠肾小管间质损伤的影响及其机制。方法采用嘌呤霉素肾病模型，以依那普利作阳性对照，观察黄芪当归合剂对肾功能和结构的影响，及其与肾组织局部肾素-血管紧张素系统(RAS)、细胞浸润、转型的关系。结果黄芪当归合剂具有与血管紧张素转换酶抑制剂(ACEI)相似的防止和减轻肾功能恶化、肾组织病理改变尤其是小管间质损伤的作用(P<0.05)。这作用与局部RAS无关，而与其抑制肾组织单核巨噬细胞的浸润(P<0.01)和肾小球系膜细胞、间质成纤维细胞转型(P<0.01)有关。结论黄芪当归合剂明显减轻小管间质损伤，这与其肾功能保护作用相一致。
, http://www.100md.com
Astragalus and Angelica retard the progressive tubulointerstitial lesions in puromycin nephropathy
YU Ling ,ZHANG Junfeng ,LI Jingzi,et al.
(Renal Division,Department of Medicine,Institute of Nephrology,First Hospital,Peking University,Beijing 100034,China)
Abstract：Objective To investigate the protective effects of Astragali and Angelica (A&A) on tubulointerstitial damage and its possible mechanisms.Methods Nephrotic syndrome (NS) with renal fibrosis was induced by peritoneal injections of puromycin.Renal function was measured at the end of the study.Pathologic changes were studied semiquantitatively.ED-1 positive cells and α-actin were assessed by immunohistochemistry.Renal tissue RAS were measured by immunoassay or colorimetry.Results A&A protected renal function,which was coincided with amelioration of pathologic lesions especially tubulointerstitial injury.This was associated with the inhibition of monocyte/macrophage infiltration and activation of interstitial fibroblasts.A&A had no effect on renal RAS.Conclusion A&A can retard the deterioration of renal function and progression of tubulointerstitial fibrosis.
, 百拇医药
Keywords：Astragali;Angelica;α-actin;Tubulointerstitum 基金项目：国家自然科学基金资助项目(39670909)
作者简介：张俊峰，全国肾脏病骨干研修班学员，现在山西省人民医院肾内科
参考文献：
[1]李丽英，王海燕，朱世乐，等.黄芪当归对肾病综合征鼠肝白蛋白的表达作用.中华医学杂志，1995，75：276-279.
[2]陈孟华，李丽英，潘辑圣，等.黄芪当归对肾病综合征大鼠肌肉蛋白质代谢的影响.中华肾脏病杂志，1997，13：153-155.
[3]李宁军，李惊子，王海燕，等.黄芪当归合剂对肾病综合征鼠脂蛋白脂酶和卵磷脂胆固醇酰基转移酶的影响.中国中西医结合杂志，1999，19：484-486.
, 百拇医药
[4]俞雷，李惊子，洪健美，等.黄芪当归合剂降低肾病综合征大鼠血脂机制的探讨.中华肾脏病杂志，1999，15：337-339.
[5]鲁盈，李惊子，郑欣，等.黄芪当归合剂对肾病综合征血清脂谱和肾小球硬化的影响.中国中西医结合杂志，1997，8：478-480.
[6]Radford MG Jr,Donadio JV Jr,Bergstralh EJ,et al.Predicting renal outcome in IgA Nephropathy.J Am Soc Nephrol,1997,8:199-207.
[7]Mizuno S,Horikawa Y,Okamoto M,Et al.Preventive effect of ACE inhibitor on interstitial myofibroblast formation and matrix deposition in a nephrotic model.Renal Failure,1998,20:481-491.
, 百拇医药
[8]Wolf G.Molecular mechanisms of angiotensin Ⅱ in the kidney:emerging role in the progression of renal disease:beyond haemodynamics.Nephrol Dial Transplant,1998,13:1131-1142.
[9]Ortega MR,Egido J.Angiotensin Ⅱ modulates cell growthrelated events and synthesis of matrix proteins in renal interstitial fibroblasts.Kidney Int,1997,52:1497-1510.
[10]Gunning ME,Ingelfinger JR,King AJ,et al.Vasoactive peptides and the kidney.In:Brenner BM,ed.The Kidney.5th ed.Philedephia:W B Saunders Company,1996.627-649.
[11]Yang N,Wu LL,Nikolic-Patenson J,et al.Local macrophage and myofibroblast proliferation in progressive renal injury in the rat remnant kiney.Nephrol Dial Transplant,1998,13:1967-1974.
[12]丁伟，李惊子，邹万忠，等.黄芪当归合剂对肾病综合征鼠肾转化生长因子β1的影响.中华肾脏病杂志，1998，14：229-232.
收稿日期：1999-11-25, 百拇医药
单位：100034 北京大学第一医院肾内科 肾脏病研究所
关键词：黄芪；当归；α-肌动蛋白；肾小管间质
中华肾脏病杂志000502摘 要：目的探讨黄芪当归合剂对肾病综合征鼠肾小管间质损伤的影响及其机制。方法采用嘌呤霉素肾病模型，以依那普利作阳性对照，观察黄芪当归合剂对肾功能和结构的影响，及其与肾组织局部肾素-血管紧张素系统(RAS)、细胞浸润、转型的关系。结果黄芪当归合剂具有与血管紧张素转换酶抑制剂(ACEI)相似的防止和减轻肾功能恶化、肾组织病理改变尤其是小管间质损伤的作用(P<0.05)。这作用与局部RAS无关，而与其抑制肾组织单核巨噬细胞的浸润(P<0.01)和肾小球系膜细胞、间质成纤维细胞转型(P<0.01)有关。结论黄芪当归合剂明显减轻小管间质损伤，这与其肾功能保护作用相一致。
, http://www.100md.com
Astragalus and Angelica retard the progressive tubulointerstitial lesions in puromycin nephropathy
YU Ling ,ZHANG Junfeng ,LI Jingzi,et al.
(Renal Division,Department of Medicine,Institute of Nephrology,First Hospital,Peking University,Beijing 100034,China)
Abstract：Objective To investigate the protective effects of Astragali and Angelica (A&A) on tubulointerstitial damage and its possible mechanisms.Methods Nephrotic syndrome (NS) with renal fibrosis was induced by peritoneal injections of puromycin.Renal function was measured at the end of the study.Pathologic changes were studied semiquantitatively.ED-1 positive cells and α-actin were assessed by immunohistochemistry.Renal tissue RAS were measured by immunoassay or colorimetry.Results A&A protected renal function,which was coincided with amelioration of pathologic lesions especially tubulointerstitial injury.This was associated with the inhibition of monocyte/macrophage infiltration and activation of interstitial fibroblasts.A&A had no effect on renal RAS.Conclusion A&A can retard the deterioration of renal function and progression of tubulointerstitial fibrosis.
, 百拇医药
Keywords：Astragali;Angelica;α-actin;Tubulointerstitum 基金项目：国家自然科学基金资助项目(39670909)
作者简介：张俊峰，全国肾脏病骨干研修班学员，现在山西省人民医院肾内科
参考文献：
[1]李丽英，王海燕，朱世乐，等.黄芪当归对肾病综合征鼠肝白蛋白的表达作用.中华医学杂志，1995，75：276-279.
[2]陈孟华，李丽英，潘辑圣，等.黄芪当归对肾病综合征大鼠肌肉蛋白质代谢的影响.中华肾脏病杂志，1997，13：153-155.
[3]李宁军，李惊子，王海燕，等.黄芪当归合剂对肾病综合征鼠脂蛋白脂酶和卵磷脂胆固醇酰基转移酶的影响.中国中西医结合杂志，1999，19：484-486.
, 百拇医药
[4]俞雷，李惊子，洪健美，等.黄芪当归合剂降低肾病综合征大鼠血脂机制的探讨.中华肾脏病杂志，1999，15：337-339.
[5]鲁盈，李惊子，郑欣，等.黄芪当归合剂对肾病综合征血清脂谱和肾小球硬化的影响.中国中西医结合杂志，1997，8：478-480.
[6]Radford MG Jr,Donadio JV Jr,Bergstralh EJ,et al.Predicting renal outcome in IgA Nephropathy.J Am Soc Nephrol,1997,8:199-207.
[7]Mizuno S,Horikawa Y,Okamoto M,Et al.Preventive effect of ACE inhibitor on interstitial myofibroblast formation and matrix deposition in a nephrotic model.Renal Failure,1998,20:481-491.
, 百拇医药
[8]Wolf G.Molecular mechanisms of angiotensin Ⅱ in the kidney:emerging role in the progression of renal disease:beyond haemodynamics.Nephrol Dial Transplant,1998,13:1131-1142.
[9]Ortega MR,Egido J.Angiotensin Ⅱ modulates cell growthrelated events and synthesis of matrix proteins in renal interstitial fibroblasts.Kidney Int,1997,52:1497-1510.
[10]Gunning ME,Ingelfinger JR,King AJ,et al.Vasoactive peptides and the kidney.In:Brenner BM,ed.The Kidney.5th ed.Philedephia:W B Saunders Company,1996.627-649.
[11]Yang N,Wu LL,Nikolic-Patenson J,et al.Local macrophage and myofibroblast proliferation in progressive renal injury in the rat remnant kiney.Nephrol Dial Transplant,1998,13:1967-1974.
[12]丁伟，李惊子，邹万忠，等.黄芪当归合剂对肾病综合征鼠肾转化生长因子β1的影响.中华肾脏病杂志，1998，14：229-232.
收稿日期：1999-11-25, 百拇医药