低密度脂蛋白及氧化修饰低密度脂蛋白对人系膜细胞增殖及基质合成的影响
作者：陶建瓴 李学旺 苏颖 段琳 林嘉友
单位：陶建瓴 李学旺 苏颖 段琳(100730 北京，中国医学科学院 中国协和医科大学北京协和医院肾内科)；林嘉友(基础医学研究所免疫室)
关键词：低密度脂蛋白；胶原Ⅳ型；肾小球硬化症；氧化修饰低密度脂蛋白；系膜细胞
中华肾脏病杂志000509摘 要：目的观察不同浓度低密度脂蛋白(LDL)及氧化修饰低密度脂蛋白(ox-LDL)对人系膜细胞(HMC)增殖和细胞毒性作用，及对培养细胞上清Ⅳ型胶原的含量及表达Ⅳ型胶原α1链mRNA的影响。方法采用体外HMC培养，H3-thymidine掺入研究人系膜细胞增殖，LDL释放实验研究细胞毒作用，ELISA法测定Ⅳ型胶原含量和逆转录-聚合酶链反应(RT-PCR)法研究Ⅳ型胶原α1链mRNA表达。结果LDL能刺激HMC增殖；低浓度ox-LDL抑制HMC增殖，高浓度ox-LDL剂量依赖性表现细胞毒性作用；LDL和低浓度ox-LDL能通过上调HMC表达Ⅳ型胶原α1链mRNA促进其产生Ⅳ型胶原。结论LDL在引起和促进肾小球硬化的发生和发展中起一定作用，LDL氧化后，可加重该作用。
, 百拇医药
Effects of LDL and ox-LDL on human mesangial cells proliferation and mesangial matrix production
TAO Jianling ,LI Xuewang ,SU Ying,et al.
(Department of Nephrology,Peking Union Medical College Hospital,Beijing 100730,China)
Abstract：Objective To observe the effects of LDL and ox-LDL on human mesangial cells(HMC)proliferation and viability,the concentrations of collagen Ⅳ in the supernatants of cultured HMC and the expression of collagen Ⅳα1 mRNA by HMC.Methods The culture of HMC was established in vitro.Cell proliferation and viability were studied by H3-thymidine incorporation and LDH releasing test respectively.The concentration of collagen Ⅳ was measured by ELISA and the expression of collagen Ⅳα1 mRNA was examined by RT-PCR.Results HMC proliferation could be stimulated by LDL and be inhibited by ox-LDL at low concentrations.Cytotoxicity was showed by ox-LDL at high concentrations in concentration-dependent manner.Production of collagen Ⅳ by HMC could be stimulated by either LDL or ox-LDL at low concentrations via promoting HMC expression of collagen Ⅳα1 mRNA.Conclusion LDL and ox-LDL may contribute to the pathogenesis of glomerulosclerosis through influencing the mesangial cell proliferation or viability and accumulation of mesangial matrix.
, http://www.100md.com
Keywords：Low density lipoprotein;Collagen type Ⅳ;Glomerulosclerosis;Oxidized low density lipoprotein;Mesangial cell;
参考文献：
[1]Kreisberg JI,Karnovsky MJ.Glomerular cells in culture.Kidney Int,1983,23:439-447.
[2]张林华,刘秉文.一次性密度梯度超速离心分离人血清脂蛋白.生物化学与生物物理学报.1989,21:257-260.
[3]王克勤,周凤兰,程小颖.琼脂糖凝胶电泳用于人血清β-脂蛋的分离分型.生物化学与生物物理学报.1979,11: 37-49.
, http://www.100md.com
[4]Moorhead JF,Chan MK,EI-Nahas M,et al.Lipid nephrotoxicity in chronic progressive glomerular and tubulointerstitial disease. Lancet,1982,2:1309-1311.
[5]Schlondorff D.Cellular mechanisms of lipid injury in the glomerulus.Am J Kidney Dis,1993,22:72-82.
[6]Kramer-Guth A,Nauck M.VLDL and LDL metabolism in human and rat mesangial cells.Nephron,1996,74:378-385.
[7]David CW,Ravinder SC,Nicholas T,et al.Oxidation of low density lipoprotein by mesangial cells may promote glomerular injury.Kidney Int,1994,45:1628-1636.
, 百拇医药
[8]Kim SB,Kang SA,Cho YJ,et al.Effects of low density lipoprotein on type Ⅳ collagen production by cultured rat mesangial cells.Nephron,1994,67:327-333.
[9]Kamanna M,Bui DT,Roh DD,et al.LDL and oxidized-LDL stimulate collagen synthesis by mesangial cells.J Am Soc Nephrol,1992,3:634.
[10]Keane WF,Kasiske BL,O’Donnell MP,et al.Oxidized low density lipoprotein increases mesangial cell expression of type Ⅳ collagen [α1(Ⅳ)] Mrna.J Am Soc Nephrol,1992:3:635.
[11]Hyun SL,Bong CK,Young Sk,et al.Involvement of oxidation in LDL-induced collagen gene regulation in mesangial cells.Kidney Int,1996,50:1582-1590.
收稿日期：1998-12-24, http://www.100md.com
单位：陶建瓴 李学旺 苏颖 段琳(100730 北京，中国医学科学院 中国协和医科大学北京协和医院肾内科)；林嘉友(基础医学研究所免疫室)
关键词：低密度脂蛋白；胶原Ⅳ型；肾小球硬化症；氧化修饰低密度脂蛋白；系膜细胞
中华肾脏病杂志000509摘 要：目的观察不同浓度低密度脂蛋白(LDL)及氧化修饰低密度脂蛋白(ox-LDL)对人系膜细胞(HMC)增殖和细胞毒性作用，及对培养细胞上清Ⅳ型胶原的含量及表达Ⅳ型胶原α1链mRNA的影响。方法采用体外HMC培养，H3-thymidine掺入研究人系膜细胞增殖，LDL释放实验研究细胞毒作用，ELISA法测定Ⅳ型胶原含量和逆转录-聚合酶链反应(RT-PCR)法研究Ⅳ型胶原α1链mRNA表达。结果LDL能刺激HMC增殖；低浓度ox-LDL抑制HMC增殖，高浓度ox-LDL剂量依赖性表现细胞毒性作用；LDL和低浓度ox-LDL能通过上调HMC表达Ⅳ型胶原α1链mRNA促进其产生Ⅳ型胶原。结论LDL在引起和促进肾小球硬化的发生和发展中起一定作用，LDL氧化后，可加重该作用。
, 百拇医药
Effects of LDL and ox-LDL on human mesangial cells proliferation and mesangial matrix production
TAO Jianling ,LI Xuewang ,SU Ying,et al.
(Department of Nephrology,Peking Union Medical College Hospital,Beijing 100730,China)
Abstract：Objective To observe the effects of LDL and ox-LDL on human mesangial cells(HMC)proliferation and viability,the concentrations of collagen Ⅳ in the supernatants of cultured HMC and the expression of collagen Ⅳα1 mRNA by HMC.Methods The culture of HMC was established in vitro.Cell proliferation and viability were studied by H3-thymidine incorporation and LDH releasing test respectively.The concentration of collagen Ⅳ was measured by ELISA and the expression of collagen Ⅳα1 mRNA was examined by RT-PCR.Results HMC proliferation could be stimulated by LDL and be inhibited by ox-LDL at low concentrations.Cytotoxicity was showed by ox-LDL at high concentrations in concentration-dependent manner.Production of collagen Ⅳ by HMC could be stimulated by either LDL or ox-LDL at low concentrations via promoting HMC expression of collagen Ⅳα1 mRNA.Conclusion LDL and ox-LDL may contribute to the pathogenesis of glomerulosclerosis through influencing the mesangial cell proliferation or viability and accumulation of mesangial matrix.
, http://www.100md.com
Keywords：Low density lipoprotein;Collagen type Ⅳ;Glomerulosclerosis;Oxidized low density lipoprotein;Mesangial cell;
参考文献：
[1]Kreisberg JI,Karnovsky MJ.Glomerular cells in culture.Kidney Int,1983,23:439-447.
[2]张林华,刘秉文.一次性密度梯度超速离心分离人血清脂蛋白.生物化学与生物物理学报.1989,21:257-260.
[3]王克勤,周凤兰,程小颖.琼脂糖凝胶电泳用于人血清β-脂蛋的分离分型.生物化学与生物物理学报.1979,11: 37-49.
, http://www.100md.com
[4]Moorhead JF,Chan MK,EI-Nahas M,et al.Lipid nephrotoxicity in chronic progressive glomerular and tubulointerstitial disease. Lancet,1982,2:1309-1311.
[5]Schlondorff D.Cellular mechanisms of lipid injury in the glomerulus.Am J Kidney Dis,1993,22:72-82.
[6]Kramer-Guth A,Nauck M.VLDL and LDL metabolism in human and rat mesangial cells.Nephron,1996,74:378-385.
[7]David CW,Ravinder SC,Nicholas T,et al.Oxidation of low density lipoprotein by mesangial cells may promote glomerular injury.Kidney Int,1994,45:1628-1636.
, 百拇医药
[8]Kim SB,Kang SA,Cho YJ,et al.Effects of low density lipoprotein on type Ⅳ collagen production by cultured rat mesangial cells.Nephron,1994,67:327-333.
[9]Kamanna M,Bui DT,Roh DD,et al.LDL and oxidized-LDL stimulate collagen synthesis by mesangial cells.J Am Soc Nephrol,1992,3:634.
[10]Keane WF,Kasiske BL,O’Donnell MP,et al.Oxidized low density lipoprotein increases mesangial cell expression of type Ⅳ collagen [α1(Ⅳ)] Mrna.J Am Soc Nephrol,1992:3:635.
[11]Hyun SL,Bong CK,Young Sk,et al.Involvement of oxidation in LDL-induced collagen gene regulation in mesangial cells.Kidney Int,1996,50:1582-1590.
收稿日期：1998-12-24, http://www.100md.com