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水飞蓟宾对大鼠肝贮脂细胞HSC-T6增殖和胶原合成的影响
http://www.100md.com 《第二军医大学学报》2000年第10期
水飞蓟宾|贮脂细胞|增殖|胶原合成
     作者:张珉 张俊平 王杰松 周斌 郭澄 谢渭芬 张纯 钱定华

    单位:张珉(第二军医大学药学院生化药学教研室,上海 200433);张俊平(第二军医大学药学院生化药学教研室,上海 200433);王杰松(第二军医大学药学院生化药学教研室,上海 200433);周斌(第二军医大学药学院生化药学教研室,上海 200433);郭澄(长征医院药学部) 谢渭芬(消化内科) 张纯(长征医院药学部);钱定华(第二军医大学药学院生化药学教研室,上海 200433)

    关键词:水飞蓟宾;贮脂细胞;增殖;胶原合成

    第二军医大学学报001009 [摘要] 目的:观察水飞蓟宾对大鼠肝贮 脂细胞HSC-T6增殖和胶原合成的影响,探讨其保护肝脏及抗硬化机制。方法:采用结晶紫染色法测定细胞增殖,3H-脯氨酸掺入法测定胶原合成。结 果:水飞蓟宾(6.25~50 μg/ml)以浓度依赖方式抑制血清、巨噬细胞条件培 养液以及血小板源生长因子或转化生长因子β1诱导的细胞增殖和胶原合成。结 论:抑制贮脂细胞增殖和胶原合成可能是水飞蓟宾保护肝脏抗硬化机制之一。

    [中图分类号] R 285.5 [文献标识码] A

    [文章编号] 0258-879X(2000)10-0932-03

    Effects of silibinin on proliferation and collagen synthesis of hepatic stellate cells, HSC-T6 in rats

    ZHANG Min, ZHANG Jun-Ping, WANG Jie-Song, ZHOU Bin,QIAN Ding-Hua

    (Department of Biochemical Pharmacy, College of Pharmacy, Second Military Medical University, Shanghai 200433, China)

    GUO Cheng,ZHANG Chun

    (Department of Pharmacy, Changzheng Hospital)

    XIE Wei-Fen

    (Department of Gas troenterology)

    [ABSTRACT] Objective:To study the hepato-protective and anti fibrotic properties of silibinin, and its effects on proliferation and collagen synthesis of immortalized rat hepatic stellate cell line, HSC-T6 cells. Methods: Cell proliferation was measured by cristal violet staining as sa y. Collagen synthesis was determined by 3 H-proline incorporation. Results: Silibinin (6.25-50 μg/ml) concentration-dependentl y reduced the increase of cell proliferation and collagen synthesis of HSC-T6 cells stimulated by serum, macrophage conditioned medium and cytokines platelet -derived growth factor or transforming growth factor β1. Conclusion: Inhibition of hepatic stellate cell proliferation and collagen synthesi s may be one important mechanism of hepatoprotective and antifibrotic properties of silibinin. ......

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