RT-PCR检测蓝舌病毒技术的建立
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武汉大学医学院医学病毒研究所 湖北武汉430071 张芃玮；董长垣；涂攀；郭淑芳；陈晓；严银芳
蓝病毒|dsRNA的RTPCR|病毒核酸检测
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武汉大学医学院医学病毒研究所 湖北武汉430071 张芃玮；董长垣；涂攀；郭淑芳；陈晓；严银芳
关键词：蓝病毒；dsRNA的RTPCR；病毒核酸检测
摘要：Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to is...
武汉大学医学院医学病毒研究所 湖北武汉430071 张芃玮；董长垣；涂攀；郭淑芳；陈晓；严银芳
关键词：蓝病毒；dsRNA的RTPCR；病毒核酸检测
摘要：Using primers complementary to the conserved sequence previously published of BTV genomic dsRNA segment 7,a part of the 5′end of segment 7 was synthesized and amplified by RT PCR method.We adapted this method to test on 12 blood samples suspected to be with BTV and the blood sample of a health sheep as a negative control.The results suggested that eleven of the twelve samples were positive,one was negative.In order to compare the sensitivity and reliability of RT PCR technique,Vero cells were used to is...
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