IL-4对重型肝炎患者外周血单个核细胞IL-1α表达调节的研究
作者：石裕明 张立煌 方海林 何南祥 刘克洲 李敏伟 姚航平
单位：310031 杭州，激，浙江 医科大学传染病研究所(石裕明，现在广州市第八人民医院传染病研究所工作)
关键词：亚急性重型肝炎；慢性重型肝炎；白细胞介素-4；白细胞介素-1α外周血单个核细胞
中华传染病杂志980306 【摘要】 目的 评价IL-4在重型肝炎治疗中的潜在应用价值。方法 应用免疫组化法和半定量RT-PCR系统地观察IL-4对重型肝炎患者PBMCs IL-1α表达的影响。结果 IL-4在蛋白质和mRNA水平明显抑制LPS诱导的亚急性和慢性重型肝炎患者PBMCs IL-1α的产生，这种抑制作用呈剂量和时间依赖的方式。IL-4于100U/ml时开始出现抑制效应，于1 000U/ml时抑制作用接近最大。虽然IL-4对慢性重型肝炎患者PBMCs IL-1α的抑制作用略低，但与未经IL-4处理的PBMCs相比较，IL-1α的表达水平仍明显下降。结论 IL-4对亚急性和慢性重型肝炎PBMCs IL-1α的表达均具有较强的下向调节作用，在重型肝炎的治疗上可能具有潜在的应用价值，值得进一步研究。
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Study on regulation of IL-4 on expression of IL-1α in PBMCs from patients with subacute and chronic severe hepatitis Shi Yuming, Zhang Lihuang, Fang Hailin, et al. Institute of Infectious Diseases,Zhejiang Medical University, Hangzhou 310031
【Abstract】 Objective To estimate the potential clinical value of IL-4 in the treatment of severe hepatitis. Methods In the present study, we observed the effect of IL-4 on expression of IL-1α in peripheral blood mononuclear cells (PBMCs) from patients with severe hepatitis at cellular and molecular levels using immunohistochemical assay and the technique of semiquantitative reverse transcriptional polymerase chain reaction (RT-PCR).The isolated PBMCs were cultured in the presence of increasing concentration of IL-4 or IL-4 was added to cultures at different time courses after the challenge of LPS. Results It was found that IL-4 in dose-and time-dependent manner down-regulated the LPS-induced production of IL-1α in PBMCs from patients with severe hepatitis at the level of protein and mRNA. The inhibitory effect of IL-4 could be observed at the concentration of 100U/ml and nearly got to the climax at 1 000U/ml.Although the suppression of IL-4 on the production of IL-1α in PBMCs from chronic severe hepatitis was slightly weaker than on that from subacute severe hepatitis,there existed significant difference when compared with the control PBMCs untreated with IL-4. Conclusion These findings showed that IL-4 was a potent down-regulator of IL-1α and might play a potential role in the treatment of severe hepatitis.The further clinical study on IL-4 remained to be done.
, 百拇医药</
单位：310031 杭州，激，浙江 医科大学传染病研究所(石裕明，现在广州市第八人民医院传染病研究所工作)
关键词：亚急性重型肝炎；慢性重型肝炎；白细胞介素-4；白细胞介素-1α外周血单个核细胞
中华传染病杂志980306 【摘要】 目的 评价IL-4在重型肝炎治疗中的潜在应用价值。方法 应用免疫组化法和半定量RT-PCR系统地观察IL-4对重型肝炎患者PBMCs IL-1α表达的影响。结果 IL-4在蛋白质和mRNA水平明显抑制LPS诱导的亚急性和慢性重型肝炎患者PBMCs IL-1α的产生，这种抑制作用呈剂量和时间依赖的方式。IL-4于100U/ml时开始出现抑制效应，于1 000U/ml时抑制作用接近最大。虽然IL-4对慢性重型肝炎患者PBMCs IL-1α的抑制作用略低，但与未经IL-4处理的PBMCs相比较，IL-1α的表达水平仍明显下降。结论 IL-4对亚急性和慢性重型肝炎PBMCs IL-1α的表达均具有较强的下向调节作用，在重型肝炎的治疗上可能具有潜在的应用价值，值得进一步研究。
, http://www.100md.com
Study on regulation of IL-4 on expression of IL-1α in PBMCs from patients with subacute and chronic severe hepatitis Shi Yuming, Zhang Lihuang, Fang Hailin, et al. Institute of Infectious Diseases,Zhejiang Medical University, Hangzhou 310031
【Abstract】 Objective To estimate the potential clinical value of IL-4 in the treatment of severe hepatitis. Methods In the present study, we observed the effect of IL-4 on expression of IL-1α in peripheral blood mononuclear cells (PBMCs) from patients with severe hepatitis at cellular and molecular levels using immunohistochemical assay and the technique of semiquantitative reverse transcriptional polymerase chain reaction (RT-PCR).The isolated PBMCs were cultured in the presence of increasing concentration of IL-4 or IL-4 was added to cultures at different time courses after the challenge of LPS. Results It was found that IL-4 in dose-and time-dependent manner down-regulated the LPS-induced production of IL-1α in PBMCs from patients with severe hepatitis at the level of protein and mRNA. The inhibitory effect of IL-4 could be observed at the concentration of 100U/ml and nearly got to the climax at 1 000U/ml.Although the suppression of IL-4 on the production of IL-1α in PBMCs from chronic severe hepatitis was slightly weaker than on that from subacute severe hepatitis,there existed significant difference when compared with the control PBMCs untreated with IL-4. Conclusion These findings showed that IL-4 was a potent down-regulator of IL-1α and might play a potential role in the treatment of severe hepatitis.The further clinical study on IL-4 remained to be done.
, 百拇医药</